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  • Primary Antibodies ›
  • GLUT4 Antibodies

Invitrogen

GLUT4 Monoclonal Antibody (1F8)

Advanced Verification
1 Published Figure
3 References
View all (30) GLUT4 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite GLUT4 Monoclonal Antibody (1F8)

  • Antibody Testing Data (2)
  • Published Figures (1)
  • Advanced Verification (1)
GLUT4 Antibody in Western Blot (WB)
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GLUT4 Antibody in Western Blot (WB)
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FIGURE: 1 / 4

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GLUT4 Antibody (MA1-83191) in WB

Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of 3T3-L1 (Lane 1), 3T3-L1 differentiated to adipocytes (Lane 2), HeLa (Lane 3), Hep G2 (Lane 4), NIH/3T3 (Lane 5), C2C12 (Lane 6), tissue extracts of Mouse heart (Lane 7), Mouse Skin (Lane 8) and Mouse Skeletal Muscle (Lane 9). The blot was probed with Anti-GLUT4 Monoclonal Antibody (1F8) (Product # MA1-83191, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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GLUT4 Antibody in Western Blot (WB)
GLUT4 Antibody in Western Blot (WB)
GLUT4 Antibody in Western Blot (WB)
GLUT4 Antibody

Product Details

MA1-83191

Applications
Tested Dilution
Publications

Western Blot (WB)

Assay-Dependent
View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent
-

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent
-

Immunocytochemistry (ICC/IF)

Assay-dependent
-

Immunoprecipitation (IP)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Pig, Rabbit, Rat

Published species

Human, Rabbit

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1F8

Immunogen

Partially purified vesicles containing insulin-responsive glucose transporter 4

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2191429

Product Specific Information

This antibody does not react with dog.

This product requires enzyme-mediated antigen retrieval prior to staining of paraffin sections. Proteinase K is recommended.

Mouse anti glucose transporter 4 antibody, clone 1F8 originally raised against rat intracellular low density microsomes (James et al.

Target Information

This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: glucose transporter 4; Glucose transporter 4 insuline-responsive; Glucose transporter 4, insuline-responsive; glucose transporter glut4; glucose transporter type 4; Glucose transporter type 4, insulin-responsive; GLUT-4; GT2; insulin responsive glucose transporter; insulin-responsive glucose transporter type 4; MGC93607; solute carrier family 2 member 4; solute carrier family 2 (facilitated glucose transporter), member 4; solute carrier family 2 , member 4; Solute carrier family 2, facilitated glucose transporter member 4

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Gene Aliases: Glut-4; GLUT4; SLC2A4

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UniProt ID: (Human) P14672, (Rat) P19357, (Mouse) P14142

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Entrez Gene ID: (Rabbit) 100037710, (Human) 6517, (Pig) 396754, (Rat) 25139, (Mouse) 20528

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Function(s)
glucose transmembrane transporter activity protein binding D-glucose transmembrane transporter activity transporter activity insulin-responsive hydrogen:glucose symporter activity transmembrane transporter activity substrate-specific transmembrane transporter activity
Process(es)
amylopectin biosynthetic process glucose transport cellular response to insulin stimulus hexose transmembrane transport glucose homeostasis response to ethanol brown fat cell differentiation transmembrane transport cellular response to hypoxia cellular response to osmotic stress carbohydrate metabolic process glucose import glucose transmembrane transport transport carbohydrate transport proton transport cation transmembrane transport
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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