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  • Fascin Antibodies

Invitrogen

Fascin Polyclonal Antibody

Advanced Verification
View all (53) Fascin antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Fascin Polyclonal Antibody

  • Antibody Testing Data (6)
  • Advanced Verification (1)
Fascin Antibody in Immunocytochemistry (ICC/IF)
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Fascin Antibody in Immunocytochemistry (ICC/IF)
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Fascin Antibody (PA5-78171) in ICC/IF

FSCN1 antibody detects FSCN1 protein at cytoplasm by immunofluorescent analysis. Sample: HCT116 cells were fixed in 100% MeOH for 5 min. Green: FSCN1 protein stained by FSCN1 antibody (Product # PA5-78171) diluted at 1:500. Blue: Hoechst 33342 staining. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Fascin Antibody in Immunocytochemistry (ICC/IF)
Fascin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Fascin Antibody in Western Blot (WB)
Fascin Antibody in Western Blot (WB)
Fascin Antibody in Western Blot (WB)
Fascin Antibody in Western Blot (WB)
Fascin Antibody

Product Details

PA5-78171

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Fascin 1. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2735775

Product Specific Information

Positive Control: HeLa

Predicted Reactivity: Rat (97%), Pig (94%), Bovine (94%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Fascin organizes filamentous actin into bundles with a minimum of 4. 1:1 actin/fascin ratio. It is probably involved in the assembly of actin filament bundles present in microspikes, membrane ruffles, and stress fibers.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 55 kDa actin-bundling protein; 55kDa actin-bundling protein; Fascin; fascin 1; Fascin homolog 1; fascin homolog 1, actin bundling protein; fascin homolog 1, actin-bundling protein; Fascin1; fscn1 {ECO:0000250|UniProtKB:Q16658}; p55; Singed; Singed (Drosophila) like (sea urchin fascin homolog like); singed-like (fascin homolog, sea urchin); Singed-like protein

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Gene Aliases: AI663989; FAN1; Fascin; fascin-1; FSCN1; HSN; p55; SNL

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UniProt ID: (Human) Q16658, (Rat) P85845, (Mouse) Q61553

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Entrez Gene ID: (Human) 6624, (Rat) 683788, (Mouse) 14086

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Function(s)
actin binding protein binding drug binding protein binding, bridging poly(A) RNA binding actin filament binding cadherin binding involved in cell-cell adhesion
Process(es)
cell-cell junction assembly cell proliferation anatomical structure morphogenesis positive regulation of lamellipodium assembly cell migration microspike assembly actin cytoskeleton organization regulation of microvillus assembly regulation of actin cytoskeleton organization establishment of apical/basal cell polarity cell motility actin filament bundle assembly positive regulation of filopodium assembly positive regulation of podosome assembly positive regulation of extracellular matrix disassembly cell-cell adhesion liver development cellular response to cell-matrix adhesion actin filament organization
It has to be done as per old AB suggested Products section.
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