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  • Primary Antibodies ›
  • Fascin Antibodies

Invitrogen

Fascin Polyclonal Antibody

View all (53) Fascin antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Fascin Polyclonal Antibody

  • Antibody Testing Data (6)
Fascin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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Fascin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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Fascin Antibody (PA5-116442) in IHC (P)

Immunohistochemical analysis of Fascin in a paraffin-embedded mouse testis tissue using a Fascin polyclonal antibody (Product #PA5-116442). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1:200 dilu... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Fascin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Fascin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Fascin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Fascin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Fascin Antibody in Western Blot (WB)
Fascin Antibody in Flow Cytometry (Flow)
Fascin Polyclonal Antibody

Product Details

PA5-116442

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Flow Cytometry (Flow)

1:50-1:100
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein within Human Fascin aa 1-140
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 0.2% BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2901074

Product Specific Information

Positive controls: SH-SY5Y, rat brain tissue, human tonsil tissue, human kidney tissue, mouse testis tissue.

Target Information

Fascin organizes filamentous actin into bundles with a minimum of 4. 1:1 actin/fascin ratio. It is probably involved in the assembly of actin filament bundles present in microspikes, membrane ruffles, and stress fibers.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 55 kDa actin-bundling protein; 55kDa actin-bundling protein; Fascin; fascin 1; Fascin homolog 1; fascin homolog 1, actin bundling protein; fascin homolog 1, actin-bundling protein; Fascin1; fscn1 {ECO:0000250|UniProtKB:Q16658}; p55; Singed; Singed (Drosophila) like (sea urchin fascin homolog like); singed-like (fascin homolog, sea urchin); Singed-like protein

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Gene Aliases: AI663989; FAN1; Fascin; fascin-1; FSCN1; HSN; p55; SNL

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UniProt ID: (Human) Q16658, (Rat) P85845, (Mouse) Q61553

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Entrez Gene ID: (Human) 6624, (Rat) 683788, (Mouse) 14086

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Function(s)
actin binding protein binding drug binding protein binding, bridging poly(A) RNA binding actin filament binding cadherin binding involved in cell-cell adhesion
Process(es)
cell-cell junction assembly cell proliferation anatomical structure morphogenesis positive regulation of lamellipodium assembly cell migration microspike assembly actin cytoskeleton organization regulation of microvillus assembly regulation of actin cytoskeleton organization establishment of apical/basal cell polarity cell motility actin filament bundle assembly positive regulation of filopodium assembly positive regulation of podosome assembly positive regulation of extracellular matrix disassembly cell-cell adhesion liver development cellular response to cell-matrix adhesion actin filament organization
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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