Invitrogen

FZD7 Polyclonal Antibody

Name: FZD7 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-33559

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View all (12) FZD7 antibodies

Datasheet

Protocols

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Datasheet

Protocols

Questions & Answers

Antibody Testing Data (7)
Advanced Verification (2)

FZD7 Antibody in Immunocytochemistry (ICC/IF)

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FZD7 Antibody (PA5-33559) in ICC/IF

Immunofluorescence analysis of Frizzled-7 was performed using 70% confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with FZD7 Polyclonal Antibody (Product # PA5-33559) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Prod... View More

FZD7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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FZD7 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Anti-FZD7 Polyclonal Antibody (Product # PA5-33559), shows expression of FZD7 in MDA-MB-231 when compared to SK-BR-3. {RE}

Immunogen image

Immunogen Image FZD7 Polyclonal Antibody

Product Details

PA5-33559

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

5-10 µg/mL

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Species Reactivity

Bat, Bovine, Dog, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic 20 amino acid peptide from N-terminal extracellular domain of human FZD7 / Frizzled 7.

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2550939

Target Information

Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD7 protein contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Fz family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif. FZD7 gene expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: frizzled 7, seven transmembrane spanning receptor; frizzled class receptor 7; frizzled family receptor 7; frizzled homolog 7; Frizzled, drosophila, homolog of, 7; Frizzled-7; Fz-7; FzE3; hFz7; mFz7; rizzled 7

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Gene Aliases: BOS_1968; Frizzled-7; Fz7; FZD7; FzE3; I79_002993

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UniProt ID: (Human) O75084, (Mouse) Q61090

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Entrez Gene ID: (Dog) 488478, (Human) 8324, (Bovine) 524255, (Mouse) 14369, (Chinese hamster) 100766658, (Rat) 100360552

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Function(s)

G-protein coupled receptor activity frizzled binding protein binding Wnt-protein binding PDZ domain binding Wnt-activated receptor activity signal transducer activity transmembrane signaling receptor activity

Process(es)

regulation of transcription, DNA-templated signal transduction cell surface receptor signaling pathway G-protein coupled receptor signaling pathway multicellular organism development negative regulation of cell-substrate adhesion skeletal muscle satellite cell maintenance involved in skeletal muscle regeneration Wnt signaling pathway stem cell population maintenance T cell differentiation in thymus substrate adhesion-dependent cell spreading regulation of catenin import into nucleus non-canonical Wnt signaling pathway non-canonical Wnt signaling pathway via JNK cascade positive regulation of phosphorylation negative regulation of ectodermal cell fate specification positive regulation of transcription, DNA-templated somatic stem cell division positive regulation of epithelial cell proliferation involved in wound healing canonical Wnt signaling pathway mesenchymal to epithelial transition neuron differentiation positive regulation of JNK cascade Wnt signaling pathway, planar cell polarity pathway cellular response to retinoic acid

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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FZD7 Polyclonal Antibody

FZD7 Antibody (PA5-33559) in ICC/IF

Product Details

PA5-33559

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

References (0)

Bioinformatics

Performance Guarantee

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