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  • FRMD6 Antibodies

Invitrogen

FRMD6 Polyclonal Antibody

View all (8) FRMD6 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite FRMD6 Polyclonal Antibody

  • Antibody Testing Data (5)
FRMD6 Antibody in Immunocytochemistry (ICC/IF)
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FRMD6 Antibody in Immunocytochemistry (ICC/IF)
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FRMD6 Antibody (PA5-143995) in ICC/IF

Immunocytochemistry analysis of FRMD6 in A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in FRMD6 Polyclonal antibody (Product # PA5-143995) using a dilution of 2 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate f... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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FRMD6 Antibody in Immunocytochemistry (ICC/IF)
FRMD6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
FRMD6 Antibody in Western Blot (WB)
FRMD6 Antibody in Flow Cytometry (Flow)
FRMD6 Antibody in Flow Cytometry (Flow)
FRMD6 Polyclonal Antibody

Product Details

PA5-143995

Applications
Tested Dilution
Publications

Western Blot (WB)

0.25-0.5 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL
-

Immunocytochemistry (ICC/IF)

2 µg/mL
-

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells
-

ELISA (ELISA)

0.1-0.5 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived human FRMD6/Willin recombinant protein (Position: M1-E95).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

0.05mg sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3075209

Product Specific Information

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Positive Control - WB: human Hela whole cell, human PC-3 whole cell, human PANC-1 whole cell, human CACO-2 whole cell, rat PC-12 whole cell, mouse Neuro-2a whole cell. IHC: human rectum cancer tissue. ICC/IF: A431 cell. Flow: A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

The protein encoded by this gene interacts with signaling pathways to coordinately regulate cell growth, cell proliferation, apoptosis, autophagy, and cell migration. This tumor suppressor also enhances retinoblastoma 1 gene expression in cancer cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 4.1 ezrin radixin moesin (FERM)-containing protein; expanded homolog; FERM domain-containing protein 163SCII; FERM domain-containing protein 6; FERM-domain-containing protein 163SCII; MGC17921; Willin

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Gene Aliases: 2610019M19Rik; 4930488L10Rik; AW212977; c14_5320; C14orf31; EX1; FRMD6; Willin

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UniProt ID: (Human) Q96NE9, (Mouse) Q8C0V9

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Entrez Gene ID: (Human) 122786, (Rat) 257646, (Mouse) 319710

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Function(s)
protein binding molecular_function
Process(es)
apical constriction regulation of actin filament-based process cellular protein localization
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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