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  • FEN1 Antibodies

OriGene

FEN1 Monoclonal Antibody (OTI1F3), TrueMAB™

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Cite FEN1 Monoclonal Antibody (OTI1F3), TrueMAB™

  • Antibody Testing Data (11)
FEN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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FEN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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FEN1 Antibody (CF802762) in IHC (P)

Immunohistochemical staining of paraffin-embedded human endometrium tissue within the normal limits using anti-FEN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802762) {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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FEN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
FEN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
FEN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
FEN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
FEN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
FEN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
FEN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
FEN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
FEN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
FEN1 Antibody in Western Blot (WB)
FEN1 Antibody in Western Blot (WB)
FEN1 Monoclonal Antibody (OTI1F3), TrueMAB™

Product Details

CF802762

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:150
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI1F3

Immunogen

Human recombinant protein fragment corresponding to amino acids 117-380 of human FEN1 produced in E.coli.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

FEN-1 (Flap Endonuclease and Five prime Exonuclease) is a 42 a protein that functions as an endonuclease that cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair and is required in cell-free DNA replication systems.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DNase IV; FEN-1; FEN1; Flap endonuclease 1; Flap structure-specific endonuclease 1; hFEN-1; Maturation factor 1; maturation factor-1; MF1

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Gene Aliases: FEN-1; FEN1; MF1; RAD2

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UniProt ID: (Human) P39748

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Entrez Gene ID: (Human) 2237

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Function(s)
magnesium ion binding DNA binding damaged DNA binding double-stranded DNA binding endonuclease activity RNA-DNA hybrid ribonuclease activity exonuclease activity protein binding double-stranded DNA exodeoxyribonuclease activity 5'-3' exonuclease activity 5'-flap endonuclease activity
Process(es)
telomere maintenance via recombination double-strand break repair via homologous recombination DNA replication DNA repair base-excision repair double-strand break repair memory UV protection DNA replication, removal of RNA primer RNA phosphodiester bond hydrolysis, endonucleolytic
It has to be done as per old AB suggested Products section.
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