Invitrogen

FEN1 Monoclonal Antibody (A1B12)

Name: FEN1 Monoclonal Antibody (A1B12)
Brand: Invitrogen
SKU: MA5-42534

View all (35) FEN1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (6)

FEN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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FEN1 Antibody (MA5-42534) in IHC (P)

Immunohistochemistry (Paraffin) analysis of paraffin-embedded human breast carcinoma tissue using FEN1 Monoclonal Antibody (Product # MA5-42534). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the FEN1 antibody at a dilu... View More

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Immunogen Image FEN1 Monoclonal Antibody (A1B12)

Product Details

MA5-42534

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

Flow Cytometry (Flow)

1:50-1:100

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

A1B12

Immunogen

Recombinant protein within human FEN1 aa 150-380

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Protein G

Storage buffer

TBS, pH 7.4, with 50% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2911675

Product Specific Information

Positive Control: MCF-7 cell lysate, K562 cell lysate, Daudi cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, human tonsil tissue, human liver carcinoma tissue, human breast carcinoma tissue, K562.

Subcellular Location: Nucleolus, nucleoplasm. Mitochondrion.

Target Information

FEN-1 (Flap Endonuclease and Five prime Exonuclease) is a 42 a protein that functions as an endonuclease that cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair and is required in cell-free DNA replication systems.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DNase IV; FEN-1; FEN-1 {ECO:0000255|HAMAP-Rule:MF_03140}; FEN1; fen1 {ECO:0000255|HAMAP-Rule:MF_03140}; Flap endonuclease 1; flap endonuclease 1 {ECO:0000255|HAMAP-Rule:MF_03140}; Flap structure-specific endonuclease 1; flap structure-specific endonuclease 1 {ECO:0000255|HAMAP-Rule:MF_03140}; hFEN-1; Maturation factor 1; maturation factor-1; MF1

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Gene Aliases: AW538437; FEN-1; FEN1; MF1; RAD2

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UniProt ID: (Human) P39748, (Rat) Q5XIP6

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Entrez Gene ID: (Human) 2237, (Mouse) 14156, (Rat) 84490

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Function(s)

magnesium ion binding DNA binding damaged DNA binding double-stranded DNA binding endonuclease activity RNA-DNA hybrid ribonuclease activity exonuclease activity protein binding double-stranded DNA exodeoxyribonuclease activity 5'-3' exonuclease activity 5'-flap endonuclease activity catalytic activity nuclease activity hydrolase activity hydrolase activity, acting on ester bonds manganese ion binding phosphoric ester hydrolase activity T/G mismatch-specific endonuclease activity retroviral 3' processing activity metal ion binding flap endonuclease activity

Process(es)

DNA metabolic process DNA replication DNA repair cellular response to DNA damage stimulus memory DNA replication, removal of RNA primer nucleic acid phosphodiester bond hydrolysis RNA phosphodiester bond hydrolysis, endonucleolytic DNA catabolic process, endonucleolytic base-excision repair telomere maintenance via recombination double-strand break repair via homologous recombination double-strand break repair UV protection

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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FEN1 Monoclonal Antibody (A1B12)

FEN1 Antibody (MA5-42534) in IHC (P)

Product Details

MA5-42534

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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