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Invitrogen

Epo Monoclonal Antibody (4F11)

2 Published Figures
3 References
View all (38) Epo antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Epo Monoclonal Antibody (4F11)

  • Antibody Testing Data (2)
  • Published Figures (2)
Epo Antibody in Immunocytochemistry (ICC/IF)
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Epo Antibody in Immunocytochemistry (ICC/IF)
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Epo Antibody (MA5-15684) in ICC/IF

Immunofluorescence analysis of GC7901 cells using EPO monoclonal antibody (Product # MA5-15684) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Epo Antibody in Immunocytochemistry (ICC/IF)
Epo Antibody in Western Blot (WB)
Epo Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Epo Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Details

MA5-15684

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:200-1:1,000
View 1 publication 1 publication

ELISA (ELISA)

1:10,000
-
Product Specifications

Species Reactivity

Human

Published species

Human, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4F11

Immunogen

Purified recombinant fragment of human EPO expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. not determined

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10987067

Product Specific Information

MA5-15684 targets EPO in indirect ELISA, IF and WB applications and shows reactivity with Human samples.

The MA5-15684 immunogen is purified recombinant fragment of human EPO expressed in E. Coli.

MA5-15684 detects EPO which has a predicted molecular weight of approximately 21kDa.

Target Information

Erythropoietin (EPO) protein is a growth factor essential for the survival, proliferation, and differentiation of erythroid precursors. EPO is not responsible for early commitment to the lineage, and instead induces the final maturation of partially differentiated erythroid cells and the production of red cell-specific proteins, such as hemoglobin. The EPO receptor (EPOR) is present in higher levels on precursor cells, and expression diminishes with cell maturation.Hepatocytes are the main source of EPO in the fetus, followed by interstitial cells of the kidney after birth and through adulthood. EPO expression is upregulated during hypoxia, and low levels of transcription have also been detected in lung and brain tissue under these conditions. Recent data suggest that EPO may also play a role in the protection of neuronal tissue from damage and initiation of repair.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: epoetin; Erythropoietin; MGC138142

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Gene Aliases: EP; EPO; MVCD2

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UniProt ID: (Human) P01588

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Entrez Gene ID: (Human) 2056

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Function(s)
erythropoietin receptor binding hormone activity protein binding protein kinase activator activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter apoptotic process acute-phase response signal transduction embryo implantation aging blood circulation positive regulation of cell proliferation response to salt stress negative regulation of calcium ion transport into cytosol positive regulation of neuron projection development peptidyl-serine phosphorylation erythrocyte differentiation activation of protein kinase activity response to lipopolysaccharide response to vitamin A response to testosterone positive regulation of activated T cell proliferation positive regulation of tyrosine phosphorylation of Stat5 protein hemoglobin biosynthetic process erythrocyte maturation response to estrogen positive regulation of DNA replication positive regulation of transcription, DNA-templated positive regulation of Ras protein signal transduction response to axon injury response to electrical stimulus response to hyperoxia regulation of transcription from RNA polymerase II promoter in response to hypoxia positive regulation of ERK1 and ERK2 cascade response to interleukin-1 cellular hyperosmotic response negative regulation of neuron death negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress negative regulation of erythrocyte apoptotic process negative regulation of cation channel activity
It has to be done as per old AB suggested Products section.
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