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Invitrogen

Epo Monoclonal Antibody (148436)

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite Epo Monoclonal Antibody (148436)

  • Antibody Testing Data (1)
Epo Antibody in Neutralization (Neu)
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Epo Antibody in Neutralization (Neu)
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Epo Antibody (MA5-23874) in Neu

Neutralization of Epo in TF‚1 human erythroleukemic cell line. Samples were incubated in Epo monoclonal antibody (Product # MA5-23874). Recombinant Mouse Erythropoietin/EPO stimulates proliferation in the TF‚1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Erythropoietin/EPO (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse Erythropoietin/EPO Monoclonal Antibody. The ND50 is typically 0.15-0.75 µg/mL. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Epo Antibody in Neutralization (Neu)
Epo Monoclonal Antibody (148436)

Product Details

MA5-23874

Applications
Tested Dilution
Publications

ELISA (ELISA)

0.5-2 µg/mL (Detection), 2-8 µg/mL (Capture)
-

Neutralization (Neu)

0.15-0.75 µg/mL
-
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

148436

Immunogen

CHO-derived recombinant mouse Erythropoietin Ala27-Arg192
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2609655

Product Specific Information

Reconstitute at 0.5 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

Erythropoietin (EPO) protein is a growth factor essential for the survival, proliferation, and differentiation of erythroid precursors. EPO is not responsible for early commitment to the lineage, and instead induces the final maturation of partially differentiated erythroid cells and the production of red cell-specific proteins, such as hemoglobin. The EPO receptor (EPOR) is present in higher levels on precursor cells, and expression diminishes with cell maturation.Hepatocytes are the main source of EPO in the fetus, followed by interstitial cells of the kidney after birth and through adulthood. EPO expression is upregulated during hypoxia, and low levels of transcription have also been detected in lung and brain tissue under these conditions. Recent data suggest that EPO may also play a role in the protection of neuronal tissue from damage and initiation of repair.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Erythropoietin; MGC138142

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Gene Aliases: Epo

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UniProt ID: (Mouse) P07321

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Entrez Gene ID: (Mouse) 13856

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Function(s)
erythropoietin receptor binding hormone activity protein kinase activator activity
Process(es)
angiogenesis response to hypoxia heart morphogenesis regulation of transcription from RNA polymerase II promoter apoptotic process embryo implantation positive regulation of cell proliferation negative regulation of calcium ion transport into cytosol peptidyl-serine phosphorylation erythrocyte differentiation negative regulation of myeloid cell apoptotic process positive regulation of tyrosine phosphorylation of Stat5 protein hemoglobin biosynthetic process negative regulation of apoptotic process erythrocyte maturation positive regulation of neuron differentiation positive regulation of DNA replication positive regulation of protein kinase activity positive regulation of transcription, DNA-templated positive regulation of Ras protein signal transduction cardiac muscle tissue morphogenesis ventricular cardiac muscle tissue morphogenesis vasculogenesis involved in coronary vascular morphogenesis visceral serous pericardium development cellular hyperosmotic response negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress negative regulation of erythrocyte apoptotic process negative regulation of cation channel activity
It has to be done as per old AB suggested Products section.
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