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  • EphB3 Antibodies

Invitrogen

EphB3 Polyclonal Antibody

View all (29) EphB3 antibodies

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Questions & Answers

Cite EphB3 Polyclonal Antibody

  • Antibody Testing Data (1)
EphB3 Antibody in Western Blot (WB)
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EphB3 Antibody in Western Blot (WB)
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EphB3 Antibody (PA5-87714) in WB

Western blot analysis of EphB3 in extracts of HeLa cells. Samples were incubated with EphB3 Polyclonal antibody (Product # PA5-87714) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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EphB3 Antibody in Western Blot (WB)
EphB3 Polyclonal Antibody

Product Details

PA5-87714

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 779-998 of human Eph receptor B3 (EPHB3) (NP_0044342)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

4.13 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2804362

Product Specific Information

Immunogen sequence: LSRFLEDDPS DPTYTSSLGG KIPIRWTAPE AIAYRKFTSA SDVWSYGIVM WEVMSYGERP YWDMSNQDVI NAVEQDYRLP PPMDCPTALH QLMLDCWVRD RNLRPKFSQI VNTLDKLIRN AASLKVIASA QSGMSQPLLD RTVPDYTTFT TVGDWLDAIK MGRYKESFVS AGFASFDLVA QMTAEDLLRI GVTLAGHQKK ILSSIQDMRL QMNQTLPVQV

Positive Samples: HeLa; Cellular Location: Cell membrane, Cell projection, Single-pass type I membrane protein, dendrite

Target Information

EPHB3 is a receptor tyrosine kinase that belongs to the ephrin receptor family. Members of the Eph family of kinases play important roles in diverse biological processes including nervous system development, angiogenesis and neural synapsis formation and maturation. The ephrin ligands and Eph receptors display reciprocal expression in vivo. Developing and adult neural tissue express nearly all of the Eph receptors and ephrin ligands. Ephs and ephrins play a significant role in angiogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Developmental kinase 5; EK2; Embryonic kinase 2; EPH-like kinase 2; EPH-like tyrosine kinase 2; EPH-like tyrosine kinase-2; Ephrin type-B receptor 3; human embryo kinase 2; mDK-5; Tyrosine-protein kinase receptor SEK-4; Tyrosine-protein kinase TYRO6

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Gene Aliases: AW456895; Cek10; EK2; EPHB3; ETK2; HEK2; MDK5; Sek4; TYRO6

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UniProt ID: (Human) P54753, (Mouse) P54754

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Entrez Gene ID: (Human) 2049, (Mouse) 13845, (Rat) 287989

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Function(s)
ephrin receptor activity ATP binding axon guidance receptor activity nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity transmembrane-ephrin receptor activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups
Process(es)
angiogenesis urogenital system development axon guidance axonal fasciculation cell migration peptidyl-tyrosine phosphorylation central nervous system projection neuron axonogenesis corpus callosum development regulation of cell-cell adhesion retinal ganglion cell axon guidance substrate adhesion-dependent cell spreading regulation of GTPase activity protein autophosphorylation ephrin receptor signaling pathway thymus development digestive tract morphogenesis regulation of axonogenesis positive regulation of synapse assembly palate development dendritic spine development dendritic spine morphogenesis protein phosphorylation signal transduction transmembrane receptor protein tyrosine kinase signaling pathway multicellular organism development nervous system development phosphorylation
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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