Invitrogen

EphA4 Polyclonal Antibody

Name: EphA4 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-29338

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Datasheet

Protocols

Questions & Answers

Antibody Testing Data (6)
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EphA4 Antibody (PA5-29338) in WB

Western blot was performed using Anti-EPHA4 Polyclonal Antibody (Product # PA5-29338) and a 109 kDa band corresponding to EPHA4 was observed across all the positive tissues (Mouse brain and Rat brain) and Caco-2 but absent in negative tissues (Mouse cerebellum and Mouse liver). Tissue extracts (30 µg lysate) of Mouse brain (Lane 1), Rat brain (Lane 2), Mouse cerebellum (Lane 3), Mouse liver (Lane 4) and membrane enriched extracts of Caco-2 (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolve... View More

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EphA4 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue tested owing to their inherent genetic constitution. Relative expression of EPHA4 was observed in Mouse brain and Rat brain in comparison to Mouse cerebellum and Mouse liver using Anti-EPHA4 polyclonal antibody (Product # PA5-29338) in Western Blot. {RE}

Product Details

PA5-29338

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human EphA4. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.97 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2546814

Product Specific Information

Recommended positive controls: HeLa, 293T, mouse brain, mouse hippocampus, rat brain, A2058, DDDDK-tagged EPHA4-transfected 293T.

Predicted reactivity: Mouse (100%), Rat (99%), Xenopus laevis (92%), Pig (99%), Chicken (96%), Rhesus Monkey (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

EPHA4 belongs to the ephrin receptor subfamily of the protein tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediated developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. EphA4 encodes a protein that binds ephrin-A ligands.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: EK8; epa4; EPH-like kinase 8; Ephrin type-A receptor 4; receptor protein-tyrosine kinase HEK8; TYRO1 protein tyrosine kinase; Tyrosine-protein kinase receptor MPK-3; Tyrosine-protein kinase receptor SEK; Tyrosine-protein kinase receptor SEK-1; Tyrosine-protein kinase TYRO1

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Gene Aliases: 2900005C20Rik; AI385584; Cek8; EPHA4; HEK8; rb; RGD1560587; SEK; Sek1; TYRO1

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UniProt ID: (Human) P54764, (Mouse) Q03137

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Entrez Gene ID: (Human) 2043, (Rat) 316539, (Mouse) 13838

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Function(s)

protein kinase activity GPI-linked ephrin receptor activity transmembrane-ephrin receptor activity protein binding ATP binding PH domain binding identical protein binding ephrin receptor binding DH domain binding nucleotide binding protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity ephrin receptor activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups

Process(es)

axon guidance adult walking behavior motor neuron axon guidance glial cell migration peptidyl-tyrosine phosphorylation corticospinal tract morphogenesis positive regulation of JUN kinase activity protein autophosphorylation ephrin receptor signaling pathway negative regulation of axon regeneration regulation of astrocyte differentiation regulation of axonogenesis positive regulation of dendrite morphogenesis regulation of dendritic spine morphogenesis nephric duct morphogenesis fasciculation of sensory neuron axon fasciculation of motor neuron axon positive regulation of Rho guanyl-nucleotide exchange factor activity protein phosphorylation cell adhesion transmembrane receptor protein tyrosine kinase signaling pathway multicellular organism development nervous system development phosphorylation regulation of GTPase activity

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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EphA4 Polyclonal Antibody

EphA4 Antibody (PA5-29338) in WB

Product Details

PA5-29338

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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