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  • Primary Antibodies ›
  • ERp57 Antibodies

Invitrogen

ERp57 Polyclonal Antibody

View all (41) ERp57 antibodies

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Cite ERp57 Polyclonal Antibody

  • Antibody Testing Data (8)
ERp57 Antibody in Immunocytochemistry (ICC/IF)
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ERp57 Antibody in Immunocytochemistry (ICC/IF)
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ERp57 Antibody (PA5-85392) in ICC/IF

ERp57 Polyclonal Antibody detects ERp57 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ERp57 protein stained by ERp57 Polyclonal Antibody (Product # PA5-85392) diluted at 1:1,000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin Polyclonal Antibody [GT114] (Product # MA5-31466) diluted at 1:1,000. Blue: Hoechst 33342 staining. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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ERp57 Antibody in Immunocytochemistry (ICC/IF)
ERp57 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ERp57 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ERp57 Antibody in Western Blot (WB)
ERp57 Antibody in Western Blot (WB)
ERp57 Antibody in Western Blot (WB)
ERp57 Antibody in Western Blot (WB)
ERp57 Antibody in Western Blot (WB)

Product Details

PA5-85392

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:10,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human ERp57. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2792534

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Mouse (92%), Rat (92%), Pig (94%), Rabbit (94%), Chicken (80%), Sheep (91%), Rhesus Monkey (98%), Chimpanzee (100%), Bovine (93%).

Positive Control: HepG2, HCT116, 293T, A431, HeLa, Neuro2A, GL261, C8D30, PC-12, Rat2.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 58 kDa glucose-regulated protein; 58 kDa microsomal protein; Disulfide isomerase ER-60; endoplasmic reticulum P58; endoplasmic reticulum protein; Endoplasmic reticulum resident protein 57; Endoplasmic reticulum resident protein 60; epididymis secretory protein Li 269; epididymis secretory sperm binding protein Li 93n; ER p57; ER protein 57; ER protein 60; ER-60 protease; ERp 57; ERp60; glucose regulated protein, 58 kDa; glucose regulated protein, 58kDa; GRP 57; GRP 58; HIP-70; oxidoreductase ERp57; p58; PDIA 3; phospholipase C, alpha; phospholipase C-alpha; PI PLC; protein disulfide isomerase associated 3; protein disulfide isomerase family A, member 3; protein disulfide isomerase-associated 3; Protein disulfide-isomerase A3; Q-2

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Gene Aliases: 58kDa; ER60; Erp; ERP57; ERP60; ERp61; GRP57; GRP58; HEL-S-269; HEL-S-93n; HsT17083; P58; PDI; PDI-Q2; PDIA3; PI-PLC; PLC[a]; Plca

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UniProt ID: (Human) P30101, (Mouse) P27773, (Rat) P11598

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Entrez Gene ID: (Human) 2923, (Mouse) 14827, (Rat) 29468

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Function(s)
protein disulfide isomerase activity cysteine-type endopeptidase activity phospholipase C activity protein binding disulfide oxidoreductase activity poly(A) RNA binding peptidase activity isomerase activity protein-disulfide reductase (glutathione) activity MHC class I protein binding
Process(es)
protein folding proteolysis response to endoplasmic reticulum stress cell redox homeostasis oxidation-reduction process cellular response to vitamin D positive regulation of protein folding positive regulation of extrinsic apoptotic signaling pathway antigen processing and presentation of peptide antigen via MHC class I protein import into nucleus protein retention in ER lumen signal transduction protein folding in endoplasmic reticulum
It has to be done as per old AB suggested Products section.
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