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  • EN1 Antibodies

Abnova

EN1 Monoclonal Antibody (3H2)

View all (9) EN1 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite EN1 Monoclonal Antibody (3H2)

  • Antibody Testing Data (2)
EN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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EN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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FIGURE: 1 / 2

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EN1 Antibody (H00002019-M04) in IHC (P)

Immunoperoxidase of monoclonal antibody to EN1 on formalin-fixed paraffin-embedded human cerebellum. Antibody concentration is 3 µg/mL. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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EN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
EN1 Antibody in ELISA (ELISA)
EN1 Monoclonal Antibody (3H2)

Product Details

H00002019-M04

Applications
Tested Dilution
Publications

Western Blot (WB)

1-5 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

3 µg/mL
-

ELISA (ELISA)

1 ng/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

3H2

Immunogen

EN1 (NP_001417, 266 a.a. approximately 392 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: SQQPLVWPAW VYCTRYSDRP SSGPRTRKLK KKKNEKEDKR PRTAFTAEQL QRLKAEFQAN RYITEQRRQT LAQELSLNES QIKIWFQNKR AKIKKATGIK NGLALHLMAQ GLYNHSTTTV QDKDESE*

Target Information

EN1 (Engrailed homolog 1) is a transcription factor expressed in embryonic ventral limb ectoderm and is essential for ventral limb patterning. En1 expression in the developing perichordal tube suggests that it may also be involved in vertebrae assembly. Loss of EN1 function in mice results in dorsal transformations of ventral paw structures and in subtle alterations along the proximal/distal limb axis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: engrailed homolog 1; HME1; Homeobox protein en-1; Homeobox protein engrailed-1; hu-En-1

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Gene Aliases: EN1

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UniProt ID: (Human) Q05925

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Entrez Gene ID: (Human) 2019

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter skeletal system development adult locomotory behavior anatomical structure morphogenesis dorsal/ventral pattern formation proximal/distal pattern formation cerebellum development midbrain development midbrain-hindbrain boundary development embryonic forelimb morphogenesis social behavior multicellular organism growth drinking behavior pigmentation negative regulation of neuron apoptotic process positive regulation of transcription from RNA polymerase II promoter neuron development motor learning dopaminergic neuron differentiation embryonic brain development
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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