Invitrogen

EN1 Polyclonal Antibody

Name: EN1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-14149

1 Published Figure

2 References

View all (9) EN1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (5)
Published Figures (1)

EN1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

EN1 Antibody (PA5-14149) in ICC/IF

Immunocytochemistry analysis of EN1 in U251 cells. Samples were incubated with EN1 polyclonal antibody (Product # PA5-14149) using a dilution of 1:25 for 1 h at 37°C followed by Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) at a dilution of 1:400 for 50 min at 37°C. Cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.1%, 10 min). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/mL, 1 h at 37°C). EN1 immunoreactivity is localized to Nucleus significant... View More

EN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

EN1 Antibody in Immunohistochemistry (IHC)

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Immunogen image

Immunogen Image EN1 Polyclonal Antibody

Product Details

PA5-14149

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:25

Immunocytochemistry (ICC/IF)

1:25

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human EN1 (Engrailed 1)

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography, Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2231168

Target Information

EN1 (Engrailed homolog 1) is a transcription factor expressed in embryonic ventral limb ectoderm and is essential for ventral limb patterning. En1 expression in the developing perichordal tube suggests that it may also be involved in vertebrae assembly. Loss of EN1 function in mice results in dorsal transformations of ventral paw structures and in subtle alterations along the proximal/distal limb axis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: engrailed homolog 1; engrailed-1; HME1; Homeobox protein en-1; Homeobox protein engrailed-1; hu-En-1; mo-En-1

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Gene Aliases: En-1; EN1; Mo-en.1

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UniProt ID: (Human) Q05925, (Mouse) P09065

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Entrez Gene ID: (Human) 2019, (Mouse) 13798

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding protein binding sequence-specific DNA binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter skeletal system development adult locomotory behavior anatomical structure morphogenesis dorsal/ventral pattern formation proximal/distal pattern formation cerebellum development midbrain development midbrain-hindbrain boundary development embryonic forelimb morphogenesis social behavior multicellular organism growth drinking behavior pigmentation negative regulation of neuron apoptotic process positive regulation of transcription from RNA polymerase II promoter neuron development motor learning dopaminergic neuron differentiation embryonic brain development regulation of transcription, DNA-templated transcription from RNA polymerase II promoter multicellular organism development regulation of gene expression neuron differentiation embryonic limb morphogenesis hindbrain development limb development

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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EN1 Polyclonal Antibody

EN1 Antibody (PA5-14149) in ICC/IF

Product Details

PA5-14149

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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