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EIF2AK2 Monoclonal Antibody (1D11)

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Datasheet
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Datasheet
Protocols
Questions & Answers
EIF2AK2 Monoclonal Antibody (1D11)

Product Details

H00005610-M02

Applications
Tested Dilution
Publications

Western Blot (WB)

1-5 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

1.5 µg/mL
-

Immunocytochemistry (ICC/IF)

10 µg/mL
-

ELISA (ELISA)

0.03 ng/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

1D11

Immunogen

EIF2AK2 (NP_002750, 1 a.a. approximately 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: MAGDLSAGFF MEELNTYRQK QGVVLKYQEL PNSGPPHDRR FTFQVIIDGR EFPEGEGRSK KEAKNAAAKL AVEILNKEKK AVSPLLLTTT NSSEGLSMGN

Target Information

EIF2AK2 (PKR) is one of 4 kinases that specifically phosphorylate Ser51 of translation initiation factor eIF2-alpha in response to various environmental stresses. EIF2AK2 plays a key role in antiviral defense mediated through its direct activation by double-stranded RNA produced by viral infection. PKR plays a key role in IFN induced-innate antiviral response, virus-induced apoptosis, cell growth & differentiation. PKR induces apoptosis by up-regulating Fas expression and mediates FADD/Caspase-8 death signaling pathway. Upon viral infection, it functions as a dual protein, sequentially activating both cell survival & cell death pathways using kinase independent and dependent strategies. PRKR regulates multiple pathways which include NF-kappaB activation, p53, p38, & PDGF signaling pathway. PKR has been implicated in tumor suppression & malignancy. To evade the antiviral effects of PKR, viruses have evolved multiple mechanisms, such as the inhibition of PKR by the non-structural protein (NS1) of the influenza virus. More recently, PKR has been implicated in several neurodegenerative diseases including Alzheimer, Huntington, and amyotrophic lateral sclerosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: double stranded RNA activated protein kinase; EC 2.7.11.1; eIF-2A protein kinase 2; eIF2a Kinase; Eukaryotic translation initiation factor 2-alpha kinase 2; Interferon-induced, double-stranded RNA-activated protein kinase; interferon-inducible elF2alpha kinase; Interferon-inducible RNA-dependent protein kinase; MGC126524; OTTHUMP00000201320; P1/eIF-2A protein kinase; p68 kinase; PKR; protein kinase R; Protein kinase RNA-activated; protein kinase, interferon-inducible double stranded RNA dependent; protein phosphatase 1, regulatory subunit 83; Tyrosine-protein kinase EIF2AK2

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Gene Aliases: EIF2AK1; EIF2AK2; PKR; PPP1R83; PRKR

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UniProt ID: (Human) P19525

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Entrez Gene ID: (Human) 5610

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Function(s)
double-stranded RNA binding protein kinase activity protein serine/threonine kinase activity eukaryotic translation initiation factor 2alpha kinase activity non-membrane spanning protein tyrosine kinase activity protein binding ATP binding protein phosphatase type 2A regulator activity poly(A) RNA binding
Process(es)
activation of MAPKK activity positive regulation of cytokine production transcription, DNA-templated translation protein phosphorylation negative regulation of cell proliferation response to mechanical stimulus response to virus response to toxic substance regulation of translational initiation by eIF2 alpha phosphorylation negative regulation of translation peptidyl-tyrosine phosphorylation evasion or tolerance by virus of host immune response endoplasmic reticulum unfolded protein response response to lipopolysaccharide positive regulation of chemokine production positive regulation of stress-activated MAPK cascade response to vitamin E negative regulation of osteoblast proliferation regulation of protein phosphatase type 2A activity cellular response to amino acid starvation response to interferon-alpha positive regulation of apoptotic process negative regulation of apoptotic process response to exogenous dsRNA negative regulation of viral genome replication innate immune response protein autophosphorylation positive regulation of NF-kappaB transcription factor activity defense response to virus regulation of NLRP3 inflammasome complex assembly positive regulation of NIK/NF-kappaB signaling regulation of hematopoietic progenitor cell differentiation regulation of hematopoietic stem cell proliferation regulation of hematopoietic stem cell differentiation
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