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  • KIF11 Antibodies

Bethyl Laboratories

EG5/KIF11 Polyclonal Antibody

View all (18) KIF11 antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite EG5/KIF11 Polyclonal Antibody

  • Antibody Testing Data (1)
EG5/KIF11 Antibody in Western Blot (WB)
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EG5/KIF11 Antibody in Western Blot (WB)
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EG5/KIF11 Antibody (A301-076A) in WB

Detection of human EG5/KIF11 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-EG5/KIF11 antibody (Product # A301-076A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. EG5/KIF11 was also immunoprecipitated by rabbit anti-EG5/KIF11 antibody A301-075A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A) a... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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EG5/KIF11 Antibody in Western Blot (WB)
EG5/KIF11 Polyclonal Antibody

Product Details

A301-076A

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000-1:10,000
-

Immunoprecipitation (IP)

2-5 µg/mg lysate
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residue 1006 and the C-terminus (residue 1056) of human kinesin-related motor protein Eg5 (kinesin family member 11).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.20 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.0 to 8.0, with 0.1% BSA

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Target Information

This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Kines; Kinesin-like protein 1; Kinesin-like protein KIF11; Kinesin-like spindle protein HKSP; Kinesin-related motor protein Eg5; Thyroid receptor-interacting protein 5; TR-interacting protein 5; TRIP-5

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Gene Aliases: EG5; HKSP; KIF11; KNSL1; MCLMR; TRIP5

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UniProt ID: (Human) P52732

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Entrez Gene ID: (Human) 3832

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Function(s)
microtubule motor activity ATP binding microtubule binding ATP-dependent microtubule motor activity, plus-end-directed protein kinase binding protein complex binding
Process(es)
retrograde vesicle-mediated transport, Golgi to ER microtubule-based movement spindle organization mitotic spindle organization chromosome segregation mitotic nuclear division mitotic centrosome separation antigen processing and presentation of exogenous peptide antigen via MHC class II regulation of mitotic centrosome separation cell division mitotic spindle assembly
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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