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  • EBP1 Antibodies

Invitrogen

EBP1 Recombinant Rabbit Monoclonal Antibody (JE35-48)

View all (23) EBP1 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite EBP1 Recombinant Rabbit Monoclonal Antibody (JE35-48)

  • Antibody Testing Data (6)
EBP1 Antibody in Immunocytochemistry (ICC/IF)
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EBP1 Antibody in Immunocytochemistry (ICC/IF)
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EBP1 Antibody (MA5-50805) in ICC/IF

Immunocytochemistry analysis of EBP1 in NIH/3T3 cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 &(8)451;, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were incubated with EBP1 recombinant monoclonal antibody (Product # MA5-50805) with a dilution of 1:50 in 2% negative goat serum overnight at 4 &(8)451;. Followed by secondary antibody Goat Anti-Rabbit IgG H&L (iFluor™ 488) at a dilution of 1:1,000. PBS instead of the pr... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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EBP1 Antibody in Immunocytochemistry (ICC/IF)
EBP1 Antibody in Immunocytochemistry (ICC/IF)
EBP1 Antibody in Immunocytochemistry (ICC/IF)
EBP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
EBP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
EBP1 Antibody in Western Blot (WB)
EBP1 Recombinant Rabbit Monoclonal Antibody (JE35-48)

Product Details

MA5-50805

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000
-

Immunocytochemistry (ICC/IF)

1:50
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JE35-48

Immunogen

Synthetic peptide within Human EBP1 aa 325-374/394.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 0.05% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3094156

Product Specific Information

Positive control: K562 cell lysate, HEK-293 cell lysate, PC-12 cell lysate, mouse cerebellum tissue, rat brain tissue, 293, NIH/3T3, PC-12.

Predicted band size: 44 kDa

Subcellular Location: Cytoplasm, Nucleus.

Target Information

This gene encodes an RNA-binding protein that is involved in growth regulation. This protein is present in pre-ribosomal ribonucleoprotein complexes and may be involved in ribosome assembly and the regulation of intermediate and late steps of rRNA processing. This protein can interact with the cytoplasmic domain of the ErbB3 receptor and may contribute to transducing growth regulatory signals. This protein is also a transcriptional co-repressor of androgen receptor-regulated genes and other cell cycle regulatory genes through its interactions with histone deacetylases. This protein has been implicated in growth inhibition and the induction of differentiation of human cancer cells. Six pseudogenes, located on chromosomes 3, 6, 9, 18, 20 and X, have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cell cycle protein p38-2G4 homolog; ErbB-3 binding protein 1; ErbB3-binding protein 1; ErbB3-binding protein Ebp1; hG4 1; hG4-1; IRES-specific cellular trans-acting factor 45 kDa; ITAF45; MGC81621; MGC94070; Mpp1; p38 2G4; proliferation-associated 2G4, 38kD; proliferation-associated 2G4, 38kDa; Proliferation-associated protein 1; Proliferation-associated protein 2G4; Protein p38-2G4; si:dz150i12.2; wu:fb19b11; wu:ft56d05; zgc:86732

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Gene Aliases: 38kDa; AA672939; EBP1; HG4-1; p38-2G4; PA2G4; Plfap

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UniProt ID: (Human) Q9UQ80, (Rat) Q6AYD3, (Mouse) P50580

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Entrez Gene ID: (Human) 5036, (Rat) 288778, (Mouse) 18813

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Function(s)
DNA binding transcription factor activity, sequence-specific DNA binding protein binding ubiquitin protein ligase binding poly(A) RNA binding RNA binding
Process(es)
transcription, DNA-templated rRNA processing regulation of translation cell cycle arrest cell proliferation negative regulation of apoptotic process positive regulation of cell differentiation negative regulation of transcription, DNA-templated regulation of transcription, DNA-templated
It has to be done as per old AB suggested Products section.
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