Bioss

Dnmt3a Polyclonal Antibody

Name: Dnmt3a Polyclonal Antibody
Brand: Bioss
SKU: BS-0497R

View all (31) DNMT3A antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (5)

Dnmt3a Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

Dnmt3a Antibody (BS-0497R) in ICC/IF

Image provided by One World Lab validation program. SH-SY5Y cells probed with Dnmt3a Polyclonal Antibody (bs-0497R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.

Dnmt3a Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Immunogen image

Immunogen Image Dnmt3a Polyclonal Antibody

Product Details

BS-0497R

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:500

Immunocytochemistry (ICC/IF)

1:50

Flow Cytometry (Flow)

1:20-1:100

ELISA (ELISA)

1:500-1:1,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide derived from human Dnmt3a, amino acids 26-100.

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA

Contains

0.02% ProClin 300

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AU015806; Cysteine methyltransferase DNMT3A; DNA (cytosine-5)-methyltransferase 3A; DNA (cytosine-5-)-methyltransferase 3 alpha; DNA cytosine methyltransferase 3A2; DNA methyltransferase 3A; DNA methyltransferase HsaIIIA; DNA methyltransferase MmuIIIA; DNA MTase HsaIIIA; DNA MTase MmuIIIA; Dnmt3a; W91664

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Gene Aliases: DNMT3A; DNMT3A2; M.HsaIIIA; MmuIIIA; TBRS

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UniProt ID: (Human) Q9Y6K1, (Mouse) O88508, (Rat) Q1LZ53

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Entrez Gene ID: (Human) 1788, (Mouse) 13435, (Rat) 444984

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Function(s)

DNA binding chromatin binding DNA (cytosine-5-)-methyltransferase activity protein binding DNA-methyltransferase activity identical protein binding unmethylated CpG binding metal ion binding DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates methyltransferase activity transferase activity

Process(es)

negative regulation of transcription from RNA polymerase II promoter DNA methylation methylation-dependent chromatin silencing regulation of gene expression by genetic imprinting transcription, DNA-templated regulation of transcription, DNA-templated spermatogenesis maintenance of DNA methylation DNA methylation on cytosine within a CG sequence positive regulation of cell death chromatin modification methylation DNA methylation on cytosine DNA methylation involved in embryo development DNA methylation involved in gamete generation hypermethylation of CpG island S-adenosylhomocysteine metabolic process S-adenosylmethioninamine metabolic process cellular response to amino acid stimulus C-5 methylation of cytosine mitotic cell cycle aging response to toxic substance response to ionizing radiation response to lead ion neuron differentiation response to estradiol response to vitamin A response to antidepressant response to cocaine negative regulation of gene expression, epigenetic cellular response to ethanol cellular response to hypoxia hepatocyte apoptotic process response to nutrient levels response to drug response to ethanol

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Dnmt3a Polyclonal Antibody

Dnmt3a Antibody (BS-0497R) in ICC/IF

Product Details

BS-0497R

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Target Information

References (0)

Bioinformatics

Performance Guarantee

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