Rockland

Delta-4 Polyclonal Antibody

Name: Delta-4 Polyclonal Antibody
Brand: Rockland
SKU: 600-401-696

View all (29) DLL4 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)

FIGURE: 1 / 4

Delta-4 Antibody (600-401-696) in ELISA

ELISA results of purified Rabbit anti-DELTA-4 Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1 µg of conjugate. The starting dilution of antibody was 5 µg/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n 611-103-122) and T... View More

Delta-4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

600-401-696

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500- 1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

10-20 µg/mL

ELISA (ELISA)

1:10,000-1:50,000

Immunoprecipitation (IP)

1:500-1:2,500

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Anti-DELTA-4 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Human DELTA-4.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

DELTA-4 affinity purified antibody is directed against human DELTA-4. A BLAST analysis was used to suggest reactivity with this protein from human based on 100% homology for the immunogen sequence. However, cross reactivity is expected with mouse, rat and chimpanzee DELTA-4 based on a 91% homology to the core 12 amino acid sequence within the 14 amino acid immunogen sequence. Cross reactivity with DELTA-4 homologues from other sources has not been determined.

Target Information

Delta-like ligand 4 (DLL4), also know as Delta-4, is a homologue of the Drosophila delta protein, and functions as a transmembrane bound ligand to the notch receptor Notch1. DLL4 is expressed in the vasculature and plays a critical role in vascular development. It is induced by vascular endothelial growth factor (VEGF), as a negative feedback regulator to regulate angiogenic sprouting and promote the formulation of a differentiated vascular network. DLL4 has been found to be strongly expressed in tumor vessels of clear-cell renal tumors and bladder cancer, and inhibition of DLL4 results in increased vascular proliferation but defective maturation. This in turn leads to a decrease in tumor growth, with no apparent toxicity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: delta 4; delta ligand 4; delta-like 4 homolog; delta-like 4 protein; Delta-like protein 4; Delta4; Drosophila Delta homolog 4; MGC126344; notch ligand delta-2; notch ligand DLL4

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Gene Aliases: AOS6; DLL4; hdelta2; UNQ1895/PRO4341

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UniProt ID: (Human) Q9NR61

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Entrez Gene ID: (Human) 54567

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Function(s)

Notch binding calcium ion binding protein binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter angiogenesis patterning of blood vessels blood vessel remodeling cardiac ventricle morphogenesis cardiac atrium morphogenesis ventricular trabecula myocardium morphogenesis pericardium morphogenesis signal transduction Notch signaling pathway Notch receptor processing visual perception blood circulation negative regulation of cell proliferation negative regulation of endothelial cell migration positive regulation of gene expression negative regulation of gene expression T cell differentiation dorsal aorta morphogenesis cellular response to vascular endothelial growth factor stimulus cellular response to fibroblast growth factor stimulus negative regulation of Notch signaling pathway positive regulation of Notch signaling pathway regulation of neurogenesis ventral spinal cord interneuron fate commitment regulation of neural retina development Notch signaling involved in heart development blood vessel lumenization negative regulation of cell migration involved in sprouting angiogenesis negative regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis positive regulation of neural precursor cell proliferation

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Delta-4 Polyclonal Antibody

Delta-4 Antibody (600-401-696) in ELISA

Product Details

600-401-696

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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