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  • Primary Antibodies ›
  • DOG-1 Antibodies

Invitrogen

DOG-1 Recombinant Rabbit Monoclonal Antibody (SP31)

4 References
View all (71) DOG-1 antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite DOG-1 Recombinant Rabbit Monoclonal Antibody (SP31)

  • Antibody Testing Data (3)
DOG-1 Antibody in Immunocytochemistry (ICC/IF)
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DOG-1 Antibody in Immunocytochemistry (ICC/IF)
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DOG-1 Antibody (MA5-14582) in ICC/IF

Immunofluorescence analysis of Dog1 was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Dog1 (SP31) Rabbit Monoclonal Antibody (Product # MA5-14582) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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DOG-1 Antibody in Immunocytochemistry (ICC/IF)
DOG-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
DOG-1 Antibody in Western Blot (WB)
DOG-1 Recombinant Rabbit Monoclonal Antibody (SP31)

Product Details

MA5-14582

Applications
Tested Dilution
Publications

Western Blot (WB)

1:250
-

Immunohistochemistry (IHC)

-
View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100
-

Immunocytochemistry (ICC/IF)

1:250
-

Flow Cytometry (Flow)

Assay-dependent
-
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

proprietary

Class

Recombinant Monoclonal

Type

Antibody

Clone

SP31

Immunogen

Synthetic peptide of human Dog1 (aa 400-500).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.057 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10988189

Product Specific Information

MA5-14582 targets Dog1 in IHC (P) applications and shows reactivity with Human samples.

The MA5-14582 immunogen is synthetic peptide of human Dog1.

Target Information

DOG-1, the novel marker, is expressed ubiquitously in gastrointestinal stromal tumors irrespective of kit or PDGFR alpha mutation status.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: anoctamin 1, calcium activated chloride channel; Anoctamin-1; Ca2+-activated Cl- channel; calcium activated chloride channel; Discovered on gastrointestinal stromal tumors protein 1; FLJ10261; oral cancer overexpressed 2; Oral cancer overexpressed protein 2; Transmembrane protein 16A; transmembrane protein 16A (eight membrane-spanning domains); Tumor-amplified and overexpressed sequence 2

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Gene Aliases: ANO1; DOG1; ORAOV2; TAOS2; TMEM16A

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UniProt ID: (Human) Q5XXA6

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Entrez Gene ID: (Human) 55107

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Function(s)
calcium activated cation channel activity intracellular calcium activated chloride channel activity voltage-gated chloride channel activity chloride channel activity protein binding iodide transmembrane transporter activity protein dimerization activity
Process(es)
cation transport chloride transport phospholipase C-activating G-protein coupled receptor signaling pathway multicellular organism development iodide transport ion transmembrane transport cellular response to heat positive regulation of insulin secretion involved in cellular response to glucose stimulus detection of temperature stimulus involved in sensory perception of pain cation transmembrane transport chloride transmembrane transport regulation of anion transmembrane transport
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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