Invitrogen

DNMT1 Monoclonal Antibody (60B1220.1), APC

View all (47) DNMT1 antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers
DNMT1 Monoclonal Antibody (60B1220.1), APC

Product Details

MA5-45291

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (IHC)

1:1,000
-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:10,000
-

Immunoprecipitation (IP)

Assay-dependent
-

ChIP assay (ChIP)

Assay-dependent
-
Product Specifications

Species Reactivity

Fish, Human, Mouse, Zebrafish

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

60B1220.1

Immunogen

Raised against a synthetic peptide corresponding to amino acids 637-650 of human DNMT1
View immunogen

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

95.64mM phosphate/2.48mM MES with 0.5M EDTA

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931745

Product Specific Information

2 µg/mL of MA5-45291 was sufficient for detection of Dnmt1 in 10 µg of mouse ES cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 180kDa. It will cross-react with mouse DNMT1.

Target Information

This gene encodes a subunit of the augmin complex, which regulates centrosome and mitotic spindle integrity, and is necessary for the completion of cytokinesis. The encoded protein was identified by interaction with ubiquitin C-terminal hydrolase 37. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: CXXC-type zinc finger protein 9; DNA (cytosine-5)-methyltransferase 1; DNA methyltransferase HsaI; DNA methyltransferase MmuI; DNA MTase HsaI; DNA MTase MmuI; Dnmt1; FLJ16293; MCMT; MGC104992

View more View less

Gene Aliases: ADCADN; AIM; cb1075; cb983; CXXC9; ddn; dmnt1; DNMT; DNMT1; Dnmt1o; fb05h01; HSN1E; m.HsaI; m.MmuI; MCMT; Met-1; Met1; MommeD2; mta; MTase; Uim; wu:fb05h01

View more View less

UniProt ID: (Human) P26358, (Mouse) P13864

View more View less

Entrez Gene ID: (Human) 1786, (Mouse) 13433, (Zebrafish) 30430

View more View less

Function(s)
DNA binding double-stranded DNA binding RNA binding DNA (cytosine-5-)-methyltransferase activity protein binding zinc ion binding methyl-CpG binding DNA-methyltransferase activity protein domain specific binding estrogen receptor binding histone deacetylase binding unmethylated CpG binding DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates promoter-specific chromatin binding chromatin binding catalytic activity transcription factor binding methyltransferase activity transferase activity metal ion binding
Process(es)
liver development lens development in camera-type eye DNA methylation determination of left/right symmetry maintenance of DNA methylation DNA methylation on cytosine within a CG sequence retina layer formation pancreas development exocrine pancreas development methylation DNA methylation on cytosine intrahepatic bile duct development regulation of DNA methylation digestive tract development cartilage development histone H3-K9 methylation retina morphogenesis in camera-type eye definitive hemopoiesis Kupffer's vesicle development C-5 methylation of cytosine negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated Ras protein signal transduction brain development aging response to heat response to toxic substance response to ionizing radiation positive regulation of gene expression response to activity gene silencing covalent chromatin modification neuron differentiation response to caffeine response to estradiol response to lipopolysaccharide response to vitamin A response to testosterone cellular response to platelet-derived growth factor stimulus response to antidepressant regulation of cell proliferation DNA hypermethylation response to ethanol negative regulation of gene expression, epigenetic S-adenosylhomocysteine metabolic process S-adenosylmethioninamine metabolic process S-adenosylmethionine metabolic process positive regulation of histone H3-K4 methylation negative regulation of histone H3-K9 methylation cellular response to amino acid stimulus cellular response to lead ion cellular response to transforming growth factor beta stimulus positive regulation of methylation-dependent chromatin silencing cellular response to nerve growth factor stimulus regulation of transcription, DNA-templated metabolic process regulation of gene expression chromatin modification negative regulation of transcription, DNA-templated
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support