Disclaimer

Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

  • Primary Antibodies ›
  • DNAJC27 Antibodies

OriGene

DNAJC27 Monoclonal Antibody (OTI8A10), TrueMAB™

View all (15) DNAJC27 antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite DNAJC27 Monoclonal Antibody (OTI8A10), TrueMAB™

  • Antibody Testing Data (1)
DNAJC27 Antibody in Western Blot (WB)
Group 53 Created with Sketch.
DNAJC27 Antibody in Western Blot (WB)
Group 53 Created with Sketch.

FIGURE: 1 / 1

{{ $ctrl.currentElement.advancedVerification.fullName }}
{{ $ctrl.currentElement.advancedVerification.text }}

DNAJC27 Antibody (CF811069) in WB

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DNAJC27 (RC205500, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-DNAJ. Positive lysates LY413917 (100 µg) and LC413917 (20 µg) can be purchased separately from OriGene. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
Published figure supplied by benchsci-logo
PMID: {{$ctrl.currentElement.benchSciPubmedId}}
View Product

{{$ctrl.videoDesc}}

{{$ctrl.videoLongDesc}}

DNAJC27 Antibody in Western Blot (WB)
DNAJC27 Monoclonal Antibody (OTI8A10), TrueMAB™

Product Details

CF811069

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

OTI8A10

Immunogen

Full length human recombinant protein of human DNAJC27 produced in HEK293T cell.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

GTPase which can activate the MEK/ERK pathway and induce cell transformation when overexpressed. May act as a nuclear scaffold for MAPK1, probably by association with MAPK1 nuclear export signal leading to enhanced ERK1/ERK2 signaling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: APAH1; DnaJ (Hsp40) homolog, subfamily C, member 27; DnaJ homolog subfamily C member 27; rab and DnaJ domain containing; Rab and DnaJ domain-containing protein; Rab-related GTP-binding protein; Ras-associated protein Rap1

View more View less

Gene Aliases: AI639580; C330021A05Rik; DNAJC27; Rabj; RABJS; RBJ

View more View less

UniProt ID: (Human) Q9NZQ0, (Mouse) Q8CFP6, (Rat) Q6IML7

View more View less

Entrez Gene ID: (Human) 51277, (Mouse) 217378, (Rat) 298859

View more View less

Function(s)
GTPase activity protein binding GTP binding nucleotide binding GDP binding
Process(es)
intracellular protein transport small GTPase mediated signal transduction Rab protein signal transduction positive regulation of MAPK cascade positive regulation of ERK1 and ERK2 cascade regulation of MAPK export from nucleus metabolic process
It has to be done as per old AB suggested Products section.
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support

Your items have has been added!


Host server : magellan-search-c7d89468c-sbmwt:80/100.66.128.142:80.
git-commit: 4db89d68e0ee702f860d53b64def1eb94897fade
git-url: https://github.com/thermofisher/magellan-search
git-branch: release/2.36.2-2025.09.39-1.0