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  • DNAJC10 Antibodies

Invitrogen

DNAJC10 Polyclonal Antibody

View all (12) DNAJC10 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite DNAJC10 Polyclonal Antibody

  • Antibody Testing Data (8)
DNAJC10 Antibody in Immunocytochemistry (ICC/IF)
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DNAJC10 Antibody in Immunocytochemistry (ICC/IF)
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DNAJC10 Antibody (PA5-143983) in ICC/IF

Immunocytochemistry analysis of DNAJC10 in A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in DNAJC10 Polyclonal antibody (Product # PA5-143983) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropria... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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DNAJC10 Antibody in Immunocytochemistry (ICC/IF)
DNAJC10 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
DNAJC10 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
DNAJC10 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
DNAJC10 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
DNAJC10 Antibody in Western Blot (WB)
DNAJC10 Antibody in Flow Cytometry (Flow)
DNAJC10 Antibody in Flow Cytometry (Flow)
DNAJC10 Polyclonal Antibody

Product Details

PA5-143983

Applications
Tested Dilution
Publications

Western Blot (WB)

0.25-0.5 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

2-5 µg/mL
-

Immunocytochemistry (ICC/IF)

5 µg/mL
-

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells
-

ELISA (ELISA)

0.1-0.5 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived human DNAJC10 recombinant protein (Position: D33-L793).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3075197

Product Specific Information

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Positive Control - WB: human HELA whole cell, human HEPG2 whole cell, human U87 whole cell, rat stomach tissue, rat RH35 whole cell, mouse stomach tissue, mouse HEPA1-6 whole cell. IHC: human breast cancer tissue, human placenta tissue, human prostate cancer tissue, human gastric cancer tissue. ICC/IF: A431 cell. Flow: HELA cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

This endoplasmic reticulum co-chaperone may play a role in protein folding and translocation across the endoplasmic reticulum membrane. DNAJC10 may act as a co-chaperone for HSPA5.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DnaJ (Hsp40) homolog, subfamily C, member 10; DnaJ homolog subfamily C member 10; Endoplasmic reticulum DNA J domain-containing protein 5; Endoplasmic reticulum DnaJ-PDI fusion protein 1; ER-resident protein ERdj5; J domain-containing PDI-like protein; J domain-containing protein disulfide isomerase-like protein; J-domain-containing protein disulfide isomerase-like protein; Macrothioredoxin; MTHr; protein disulfide isomerase family A, member 19

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Gene Aliases: 1200006L06Rik; D2Ertd706e; DNAJC10; ERDJ5; JPDI; MTHr; PDIA19; UNQ495/PRO1012

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UniProt ID: (Human) Q8IXB1, (Rat) Q498R3, (Mouse) Q9DC23

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Entrez Gene ID: (Human) 54431, (Rat) 295690, (Mouse) 66861

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Function(s)
ATPase activator activity protein binding protein disulfide oxidoreductase activity disulfide oxidoreductase activity oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor Hsp70 protein binding chaperone binding ATPase binding misfolded protein binding peptide-methionine (S)-S-oxide reductase activity oxidoreductase activity peptide-methionine (R)-S-oxide reductase activity methionine-R-sulfoxide reductase activity
Process(es)
negative regulation of protein phosphorylation ER-associated ubiquitin-dependent protein catabolic process positive regulation of ATPase activity protein folding in endoplasmic reticulum response to endoplasmic reticulum stress cell redox homeostasis oxidation-reduction process intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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