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  • DNA Ligase I Antibodies

Invitrogen

DNA Ligase I Recombinant Rabbit Monoclonal Antibody (7B2)

View all (18) DNA Ligase I antibodies

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Datasheet
Protocols
Questions & Answers

Cite DNA Ligase I Recombinant Rabbit Monoclonal Antibody (7B2)

  • Antibody Testing Data (3)
DNA Ligase I Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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DNA Ligase I Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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DNA Ligase I Antibody (MA5-38384) in IHC (P)

Immunohistochemistry (Paraffin) analysis of DNA Ligase I in paraffin-embedded human placenta tissue using DNA Ligase I Monoclonal Antibody (Product # MA5-38384) diluted at 1:100. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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DNA Ligase I Antibody in Immunohistochemistry (Paraffin) (IHC (P))
DNA Ligase I Antibody in Immunohistochemistry (Paraffin) (IHC (P))
DNA Ligase I Antibody in Western Blot (WB)
DNA Ligase I Recombinant Rabbit Monoclonal Antibody (7B2)

Product Details

MA5-38384

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:5,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

7B2

Immunogen

A synthesized peptide derived from human DNA Ligase I
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol, 150mM NaCl

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2898298

Product Specific Information

This antibody has been tested in direct-ELISA

Target Information

The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. There are three alternatively spliced transcript variants encoding the same protein described for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DNA ligase 1; DNA ligase I; ligase 1; ligase I, DNA, ATP-dependent; MGC117397; MGC130025; Polydeoxyribonucleotide synthase [ATP] 1

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Gene Aliases: LIG1

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UniProt ID: (Human) P18858

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Entrez Gene ID: (Human) 3978

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Function(s)
DNA binding DNA ligase activity DNA ligase (ATP) activity ATP binding metal ion binding
Process(es)
telomere maintenance via recombination DNA metabolic process DNA repair transcription-coupled nucleotide-excision repair base-excision repair nucleotide-excision repair, DNA gap filling mismatch repair anatomical structure morphogenesis V(D)J recombination DNA ligation involved in DNA repair cell division DNA biosynthetic process Okazaki fragment processing involved in mitotic DNA replication
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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