Disclaimer

Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

  • Primary Antibodies ›
  • DNA Ligase I Antibodies

Invitrogen

DNA Ligase I Recombinant Rabbit Monoclonal Antibody (0D6M4)

View all (18) DNA Ligase I antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite DNA Ligase I Recombinant Rabbit Monoclonal Antibody (0D6M4)

  • Antibody Testing Data (6)
DNA Ligase I Antibody in Immunocytochemistry (ICC/IF)
Group 53 Created with Sketch.
DNA Ligase I Antibody in Immunocytochemistry (ICC/IF)
Group 53 Created with Sketch.

FIGURE: 1 / 6

{{ $ctrl.currentElement.advancedVerification.fullName }}
{{ $ctrl.currentElement.advancedVerification.text }}

DNA Ligase I Antibody (MA5-42920) in ICC/IF

Immunofluorescence analysis of DNA Ligase I in U-2 OS cells. Samples were incubated with DNA Ligase I Monoclonal antibody (Product # MA5-42920) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
Published figure supplied by benchsci-logo
PMID: {{$ctrl.currentElement.benchSciPubmedId}}
View Product

{{$ctrl.videoDesc}}

{{$ctrl.videoLongDesc}}

DNA Ligase I Antibody in Immunocytochemistry (ICC/IF)
DNA Ligase I Antibody in Immunocytochemistry (ICC/IF)
DNA Ligase I Antibody in Immunohistochemistry (Paraffin) (IHC (P))
DNA Ligase I Antibody in Immunohistochemistry (Paraffin) (IHC (P))
DNA Ligase I Antibody in Immunohistochemistry (Paraffin) (IHC (P))
DNA Ligase I Antibody in Western Blot (WB)
DNA Ligase I Recombinant Rabbit Monoclonal Antibody (0D6M4)

Product Details

MA5-42920

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

0D6M4

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-91 of human DNA Ligase I (P18858).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2912061

Product Specific Information

Positive test controls include: Jurkat.

Immunogen sequence: MQRSIMSFFH PKKEGKAKKP EKEASNSSRE TEPPPKAALK EWNGVVSESD SPVKRPGRKA ARVLGSEGEE EDEALSPAKG QKPALDCSQV S

Target Information

The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. There are three alternatively spliced transcript variants encoding the same protein described for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: DNA ligase (ATP) 1; DNA ligase 1; DNA ligase I; ligase 1; ligase I, DNA, ATP-dependent; MGC117397; MGC130025; Polydeoxyribonucleotide synthase [ATP] 1

View more View less

Gene Aliases: AL033288; Lig-1; LIG1; LigI

View more View less

UniProt ID: (Human) P18858, (Mouse) P37913

View more View less

Entrez Gene ID: (Human) 3978, (Mouse) 16881, (Rat) 81513

View more View less

Function(s)
DNA binding DNA ligase activity DNA ligase (ATP) activity ATP binding metal ion binding nucleotide binding ligase activity
Process(es)
telomere maintenance via recombination DNA metabolic process DNA repair transcription-coupled nucleotide-excision repair base-excision repair nucleotide-excision repair, DNA gap filling mismatch repair anatomical structure morphogenesis V(D)J recombination DNA ligation involved in DNA repair cell division DNA biosynthetic process Okazaki fragment processing involved in mitotic DNA replication DNA replication DNA ligation lagging strand elongation double-strand break repair via nonhomologous end joining DNA recombination cellular response to DNA damage stimulus cell cycle response to hydrogen peroxide removal of RNA primer involved in mitotic DNA replication nucleotide-excision repair
It has to be done as per old AB suggested Products section.
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support

Your items have has been added!


Host server : magellan-search-55c564d944-rwwh4:80/100.66.128.142:80.
git-commit: 3092084cb0e494f0e07f07633350183de7fa10a8
git-url: https://github.com/thermofisher/magellan-search
git-branch: release/2.36.2-2025.09.39-1.0