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  • Primary Antibodies ›
  • DNA Ligase I Antibodies

Invitrogen

DNA Ligase I Polyclonal Antibody

View all (18) DNA Ligase I antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite DNA Ligase I Polyclonal Antibody

  • Antibody Testing Data (5)
DNA Ligase I Antibody in Immunocytochemistry (ICC/IF)
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DNA Ligase I Antibody in Immunocytochemistry (ICC/IF)
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FIGURE: 1 / 5

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DNA Ligase I Antibody (PA5-95963) in ICC/IF

Immunofluorescence analysis of DNA Ligase I in MCF-7 cells. Samples were incubated with DNA Ligase I Polyclonal antibody (Product # PA5-95963). Blue: DAPI for nuclear staining. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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DNA Ligase I Antibody in Immunocytochemistry (ICC/IF)
DNA Ligase I Antibody in Immunocytochemistry (ICC/IF)
DNA Ligase I Antibody in Western Blot (WB)
DNA Ligase I Antibody in Western Blot (WB)
DNA Ligase I Antibody in Western Blot (WB)
DNA Ligase I Polyclonal Antibody

Product Details

PA5-95963

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 565-790 of human LIG1 (NP_0002251)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.08 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2807765

Product Specific Information

Immunogen sequence: CEYKYDGQRA QIHALEGGEV KIFSRNQEDN TGKYPDIISR IPKIKLPSVT SFILDTEAVA WDREKKQIQP FQVLTTRKRK EVDASEIQVQ VCLYAFDLIY LNGESLVREP LSRRRQLLRE NFVETEGEFV FATSLDTKDI EQIAEFLEQS VKDSCEGLMV KTLDVDATYE IAKRSHNWLK LKKDYLDGVG DTLDLVVIGA YLGRGKRAGR YGGFLLASYD EDSEEL; Positive Samples: MCF7, A-549, K-562, Jurkat, 22Rv1, Mouse testis; Cellular Location: Nucleus

Target Information

The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. There are three alternatively spliced transcript variants encoding the same protein described for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DNA ligase 1; DNA ligase I; ligase 1; ligase I, DNA, ATP-dependent; MGC117397; MGC130025; Polydeoxyribonucleotide synthase [ATP] 1

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Gene Aliases: AL033288; Lig-1; LIG1; LigI

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UniProt ID: (Human) P18858, (Mouse) P37913

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Entrez Gene ID: (Human) 3978, (Mouse) 16881

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Function(s)
DNA binding DNA ligase activity DNA ligase (ATP) activity ATP binding metal ion binding nucleotide binding ligase activity
Process(es)
telomere maintenance via recombination DNA metabolic process DNA repair transcription-coupled nucleotide-excision repair base-excision repair nucleotide-excision repair, DNA gap filling mismatch repair anatomical structure morphogenesis V(D)J recombination DNA ligation involved in DNA repair cell division DNA biosynthetic process Okazaki fragment processing involved in mitotic DNA replication DNA replication DNA ligation lagging strand elongation double-strand break repair via nonhomologous end joining DNA recombination cellular response to DNA damage stimulus cell cycle response to hydrogen peroxide removal of RNA primer involved in mitotic DNA replication
It has to be done as per old AB suggested Products section.
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