Abnova

DKK1 Monoclonal Antibody (3G3)

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers
DKK1 Monoclonal Antibody (3G3)

Product Details

H00022943-M05

Applications
Tested Dilution
Publications

Western Blot (WB)

1-5 µg/mL
-

ELISA (ELISA)

10 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

3G3

Immunogen

DKK1 (NP_036374, 81 a.a. approximately 180 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.45 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

Product may be used with Western Blot (Cell lysate).

Immunogen sequence: QPYPCAEDEE CGTDEYCASP TRGGDAGVQI CLACRKRRKR CMRHAMCCPG NYCKNGICVS SDQNHFRGEI EETITESFGN DHSTLDGYSR RTTLSSKMYH

Target Information

DKK-1 is a member of the DKK protein family which also includes DKK-2, DKK-3 and DKK-4. DKK-1 was originally identified as a Xenopus head-forming molecule that behaves as an antagonist for Wnt signaling. Subsequent studies have shown that DKK-1 and DKK-4 play important regulatory roles in the Wnt/beta-catenin signaling pathway by forming inhibitory complexes with LDL receptor-related proteins 5 and 6 (LRP5 and LRP6), which are essential components of the Wnt/beta-catenin signaling system. LRP5 and LRP6 are single-pass transmembrane proteins that appear to act as co-receptors for Wnt ligands involved in the Wnt/beta-catenin signaling cascade. It has been suggested that by inhibiting Wnt/beta-catenin signaling, which is essential for posterior patterning in vertebrates, DKK-1 permits anterior development. This notion is supported by the finding that mice deficient of DKK-1 expression lack head formation and die during embryogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: dickkopf 1 homolog; dickkopf related protein-1; Dickkopf-1; dickkopf-1 like; dickkopf-like protein 1; Dickkopf-related protein 1; Dickkopf1; hDkk-1; hDkk1; SK

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Gene Aliases: DKK-1; DKK1; SK; UNQ492/PRO1008

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UniProt ID: (Human) O94907

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Entrez Gene ID: (Human) 22943

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Function(s)
signal transducer activity protein binding growth factor activity co-receptor binding receptor antagonist activity low-density lipoprotein particle receptor binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter cell morphogenesis involved in differentiation endoderm formation mesoderm formation hair follicle development regulation of receptor internalization negative regulation of Wnt signaling pathway embryonic limb morphogenesis negative regulation of BMP signaling pathway forebrain development negative regulation of protein binding response to retinoic acid negative regulation of peptidyl-serine phosphorylation negative regulation of mesodermal cell fate specification regulation of endodermal cell fate specification negative regulation of skeletal muscle tissue development negative regulation of synapse assembly regulation of synaptic transmission, glutamatergic face morphogenesis negative regulation of pathway-restricted SMAD protein phosphorylation motor learning positive regulation of heart induction by negative regulation of canonical Wnt signaling pathway negative regulation of canonical Wnt signaling pathway Wnt signaling pathway involved in somitogenesis extracellular negative regulation of signal transduction negative regulation of canonical Wnt signaling pathway involved in cardiac muscle cell fate commitment regulation of dopaminergic neuron differentiation midbrain morphogenesis negative regulation of Wnt-Frizzled-LRP5/6 complex assembly negative regulation of cardiac muscle cell differentiation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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