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  • Primary Antibodies ›
  • DRD1 Antibodies

Alomone Labs, LTD

D1 Dopamine Receptor Polyclonal Antibody

View all (30) DRD1 antibodies

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Datasheet
Protocols
Questions & Answers

Cite D1 Dopamine Receptor Polyclonal Antibody

  • Antibody Testing Data (3)
D1 Dopamine Receptor Antibody in Immunohistochemistry (Frozen) (IHC (F))
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D1 Dopamine Receptor Antibody in Immunohistochemistry (Frozen) (IHC (F))
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D1 Dopamine Receptor Antibody (ADR-001-GP200UL) in IHC (F)

Expression of DRD1in rat cortex - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Guinea pig Anti-D1Dopamine Receptor Antibody (#ADR-001-GP), (1:300), followed by goat- Anti-guinea pig-Cy3 Antibody . DRD1staining (red) appears in neuronal soma (arrows). Nuclei are stained with DAPI (blue). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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D1 Dopamine Receptor Antibody in Immunohistochemistry (Frozen) (IHC (F))
D1 Dopamine Receptor Antibody in Western Blot (WB)
D1 Dopamine Receptor Antibody in Western Blot (WB)
D1 Dopamine Receptor Polyclonal Antibody

Product Details

ADR-001-GP200UL

Applications
Tested Dilution
Publications

Western Blot (WB)

1:400
-

Immunohistochemistry (Frozen) (IHC (F))

1:300
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Guinea pig / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SSHHEPRGSISKDC, corresponding to amino acid residues 372-385 of rat DRD1, Intracellular, C-terminus
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.8 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Information

The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: D(1A) dopamine receptor; D1 receptor; D1A dopamine receptor; D1a receptor; Dopamine 1 receptor; Dopamine D1 receptor; dopamine receptor D1A; Dopamine-1A receptor; G protein-coupled receptor DRD1

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Gene Aliases: C030036C15Rik; D1a; DADR; Drd-1; DRD1; DRD1A; Gpcr15

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UniProt ID: (Human) P21728, (Mouse) Q61616

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Entrez Gene ID: (Human) 1812, (Mouse) 13488, (Rat) 24316

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Function(s)
dopamine neurotransmitter receptor activity, coupled via Gs G-protein alpha-subunit binding dopamine neurotransmitter receptor activity protein binding drug binding protein phosphatase binding angiotensin receptor binding D3 dopamine receptor binding protein complex binding dopamine binding protein heterodimerization activity ATPase binding signal transducer activity G-protein coupled receptor activity receptor binding G-protein coupled amine receptor activity
Process(es)
temperature homeostasis conditioned taste aversion behavioral fear response synaptic transmission, dopaminergic response to amphetamine negative regulation of protein kinase activity protein import into nucleus G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger adenylate cyclase-activating G-protein coupled receptor signaling pathway activation of adenylate cyclase activity adenylate cyclase-activating dopamine receptor signaling pathway synapse assembly learning memory mating behavior grooming behavior adult walking behavior visual learning positive regulation of adenylate cyclase activity involved in G-protein coupled receptor signaling pathway astrocyte development response to activity dopamine transport transmission of nerve impulse neuronal action potential regulation of vasoconstriction calcium-mediated signaling dentate gyrus development striatum development orbitofrontal cortex development cerebral cortex GABAergic interneuron migration positive regulation of cell migration negative regulation of cell migration peristalsis positive regulation of cAMP biosynthetic process response to food response to estradiol response to retinoic acid cellular response to insulin stimulus response to nicotine operant conditioning social behavior response to antidepressant regulation of dopamine metabolic process vasodilation negative regulation of circadian sleep/wake cycle, sleep dopamine metabolic process response to drug maternal behavior response to amino acid positive regulation of potassium ion transport response to morphine response to ethanol positive regulation of membrane potential glucose import habituation sensitization behavioral response to cocaine regulation of long-term neuronal synaptic plasticity response to steroid hormone positive regulation of release of sequestered calcium ion into cytosol positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway regulation of dopamine uptake involved in synaptic transmission positive regulation of synaptic transmission, glutamatergic prepulse inhibition phospholipase C-activating dopamine receptor signaling pathway long-term synaptic potentiation long term synaptic depression cellular response to hypoxia cellular response to catecholamine stimulus positive regulation of long-term synaptic potentiation positive regulation of feeding behavior startle response intracellular protein transport muscle contraction signal transduction G-protein coupled receptor signaling pathway dopamine receptor signaling pathway chemical synaptic transmission sensory perception of chemical stimulus locomotory behavior feeding behavior associative learning hippocampus development phosphatidylinositol catabolic process response to cocaine regulation of ion transport positive regulation of adenylate cyclase activity phosphatidylinositol metabolic process response to organonitrogen compound response to organic cyclic compound
It has to be done as per old AB suggested Products section.
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