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  • Primary Antibodies ›
  • Cystatin C Antibodies

Invitrogen

Cystatin C Recombinant Rabbit Monoclonal Antibody (0Z4G3)

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Datasheet
Protocols
Questions & Answers

Cite Cystatin C Recombinant Rabbit Monoclonal Antibody (0Z4G3)

  • Antibody Testing Data (2)
Cystatin C Antibody in Western Blot (WB)
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Cystatin C Antibody in Western Blot (WB)
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Cystatin C Antibody (MA5-35738) in WB

Western blot analysis of Cystatin C in extracts of various cell lines. Samples were incubated with Cystatin C Monoclonal antibody (Product # MA5-35738) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Cystatin C Antibody in Western Blot (WB)
Cystatin C Antibody in Western Blot (WB)
Cystatin C Recombinant Rabbit Monoclonal Antibody (0Z4G3)

Product Details

MA5-35738

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

0Z4G3

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-146 of human Cystatin C (P01034)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.16 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2849638

Product Specific Information

Immunogen sequence: MAGPLRAPLL LLAILAVALA VSPAAGSSPG KPPRLVGGPM DASVEEEGVR RALDFAVGEY NKASNDMYHS RALQVVRARK QIVAGVNYFL DVELGRTTCT KTQPNLDNCP FHDQPHLKRK AFCSFQIYAV PWQGTMTLSK STCQDA

Target Information

Cystatin C, a member of the type 2 cystatin superfamily, is a potent inhibitor of cysteine proteinases, both endogenous (lysosomal cathepsins), and pathogenic. Cystatin C is a 13 kDa secreted protein which is found in high concentrations in biological fluids and is thought to be expressed by all human nucleated cells. Changes in Cystatin C have been documented in a number of cardiovascular diseases, including atherosclerosis, amyloid angiopathy and myocardial infarction. Its low molecular weight also means that Cystatin C can be used as a marker of renal failure.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AD8; bA218C14.4 (cystatin C); cystatin 3; Cystatin-3; Cystatin-C; Cystatin3; CystatinC; epididymis secretory protein Li 2; Gamma-trace; MGC117328; Neuroendocrine basic polypeptide; OTTHUMP00000030440; OTTHUMP00000164181; OTTHUMP00000164182; OTTMUSP00000016736; Post-gamma-globulin; RP23-250M11.9

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Gene Aliases: ARMD11; CST3; CysC; HEL-S-2

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UniProt ID: (Human) P01034, (Mouse) P21460

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Entrez Gene ID: (Human) 1471, (Mouse) 13010

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Function(s)
beta-amyloid binding protease binding endopeptidase inhibitor activity cysteine-type endopeptidase inhibitor activity protein binding identical protein binding peptidase inhibitor activity
Process(es)
defense response response to oxidative stress positive regulation of cell proliferation negative regulation of peptidase activity negative regulation of endopeptidase activity regulation of programmed cell death extracellular fibril organization positive regulation of DNA replication negative regulation of proteolysis negative regulation of elastin catabolic process negative regulation of cell death negative regulation of collagen catabolic process negative regulation of extracellular matrix disassembly regulation of tissue remodeling cellular protein metabolic process negative regulation of blood vessel remodeling
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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