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  • Primary Antibodies ›
  • Cystatin C Antibodies

Invitrogen

Cystatin C Polyclonal Antibody

View all (81) Cystatin C antibodies

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Protocols
Questions & Answers

Cite Cystatin C Polyclonal Antibody

  • Antibody Testing Data (3)
Cystatin C Antibody in Immunohistochemistry (Frozen) (IHC (F))
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Cystatin C Antibody in Immunohistochemistry (Frozen) (IHC (F))
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Cystatin C Antibody (PA5-46963) in IHC (F)

Immunohistochemical analysis of Cystatin C in perfusion fixed frozen sections of rat brain (cerebellum). Samples were incubated in Cystatin C polyclonal antibody (Product # PA5-46963) using a dilution of 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neurons in the ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Cystatin C Antibody in Immunohistochemistry (Frozen) (IHC (F))
Cystatin C Antibody in Western Blot (WB)
Cystatin C Antibody in Western Blot (WB)
Cystatin C Polyclonal Antibody

Product Details

PA5-46963

Applications
Tested Dilution
Publications

Western Blot (WB)

1-10 µg/mL
-

Immunohistochemistry (Frozen) (IHC (F))

5-15 µg/mL
-

Immunoprecipitation (IP)

25 µg/mL
-
Product Specifications

Species Reactivity

Rat

Host/Isotype

Sheep / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat Cystatin C Met1-Ala140
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2609410

Product Specific Information

In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse Cystatin C and approximately 15% cross-reactivity with recombinant human Cystatin C is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

Cystatin C, a member of the type 2 cystatin superfamily, is a potent inhibitor of cysteine proteinases, both endogenous (lysosomal cathepsins), and pathogenic. Cystatin C is a 13 kDa secreted protein which is found in high concentrations in biological fluids and is thought to be expressed by all human nucleated cells. Changes in Cystatin C have been documented in a number of cardiovascular diseases, including atherosclerosis, amyloid angiopathy and myocardial infarction. Its low molecular weight also means that Cystatin C can be used as a marker of renal failure.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AD8; cystatin 3; Cystatin C (cysteine proteinase inhibitor); Cystatin-3; Cystatin-C; Cystatin3; CystatinC; MGC117328; OTTHUMP00000030440; OTTHUMP00000164181; OTTHUMP00000164182; OTTMUSP00000016736; RP23-250M11.9

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Gene Aliases: Cst3; CYSC

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UniProt ID: (Rat) P14841

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Entrez Gene ID: (Rat) 25307

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Function(s)
beta-amyloid binding protease binding endopeptidase inhibitor activity cysteine-type endopeptidase inhibitor activity peptidase inhibitor activity
Process(es)
eye development response to hypoxia cell activation apoptotic process defense response response to oxidative stress brain development salivary gland development embryo implantation positive regulation of cell proliferation male gonad development response to toxic substance response to carbohydrate response to inorganic substance negative regulation of peptidase activity negative regulation of collagen catabolic process negative regulation of extracellular matrix disassembly negative regulation of endopeptidase activity response to organic cyclic compound response to nutrient levels response to estradiol regulation of tissue remodeling cellular response to oxidative stress response to drug circadian sleep/wake cycle, REM sleep regulation of programmed cell death extracellular fibril organization positive regulation of DNA replication negative regulation of proteolysis response to axon injury Sertoli cell development negative regulation of elastin catabolic process negative regulation of blood vessel remodeling negative regulation of cell death cellular response to hydrogen peroxide
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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