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  • Primary Antibodies ›
  • Cystatin C Antibodies

Invitrogen

Cystatin C Polyclonal Antibody

View all (81) Cystatin C antibodies

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Protocols
Questions & Answers

Cite Cystatin C Polyclonal Antibody

  • Antibody Testing Data (3)
Cystatin C Antibody in Immunohistochemistry (Frozen) (IHC (F))
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Cystatin C Antibody in Immunohistochemistry (Frozen) (IHC (F))
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Cystatin C Antibody (PA5-46906) in IHC (F)

Immunohistochemical analysis of Cystatin C in immersion fixed frozen sections of mouse spleen. Samples were incubated in Cystatin C polyclonal antibody (Product # PA5-46906) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Cystatin C Antibody in Immunohistochemistry (Frozen) (IHC (F))
Cystatin C Antibody in Western Blot (WB)
Cystatin C Antibody in Western Blot (WB)
Cystatin C Polyclonal Antibody

Product Details

PA5-46906

Applications
Tested Dilution
Publications

Western Blot (WB)

1 µg/mL
-

Immunohistochemistry (Frozen) (IHC (F))

5-15 µg/mL
-

ELISA (ELISA)

0.1-0.4 µg/mL (Detection), 0.2-0.8 µg/mL (Capture)
-

Immunoprecipitation (IP)

25 µg/mL
-
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Cystatin C Ala21-Ala140
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2609209

Product Specific Information

In sandwich ELISAs, approximately 10% cross-reactivity with recombinant human (rh) Cystatin D is observed and less than 2% cross-reactivity with rhCystatin S/A, rhCystatin A, recombinant mouse (rm) Cystatin B, rhCystatin S/N, and rmCystatin E/M is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

Cystatin C, a member of the type 2 cystatin superfamily, is a potent inhibitor of cysteine proteinases, both endogenous (lysosomal cathepsins), and pathogenic. Cystatin C is a 13 kDa secreted protein which is found in high concentrations in biological fluids and is thought to be expressed by all human nucleated cells. Changes in Cystatin C have been documented in a number of cardiovascular diseases, including atherosclerosis, amyloid angiopathy and myocardial infarction. Its low molecular weight also means that Cystatin C can be used as a marker of renal failure.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AD8; cystatin 3; Cystatin-3; Cystatin-C; Cystatin3; CystatinC; MGC117328; OTTHUMP00000030440; OTTHUMP00000164181; OTTHUMP00000164182; OTTMUSP00000016736; RP23-250M11.9

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Gene Aliases: Cst3; CysC

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UniProt ID: (Mouse) P21460

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Entrez Gene ID: (Mouse) 13010

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Function(s)
beta-amyloid binding protease binding endopeptidase inhibitor activity cysteine-type endopeptidase inhibitor activity peptidase inhibitor activity identical protein binding
Process(es)
defense response response to oxidative stress positive regulation of cell proliferation negative regulation of peptidase activity negative regulation of endopeptidase activity regulation of programmed cell death extracellular fibril organization positive regulation of DNA replication negative regulation of proteolysis negative regulation of elastin catabolic process negative regulation of cell death
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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