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  • Creatine Kinase BB Antibodies

Invitrogen

Creatine Kinase BB Polyclonal Antibody

View all (53) Creatine Kinase BB antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite Creatine Kinase BB Polyclonal Antibody

  • Antibody Testing Data (2)
Creatine Kinase BB Antibody in Western Blot (WB)
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Creatine Kinase BB Antibody in Western Blot (WB)
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Creatine Kinase BB Antibody (PA5-80476) in WB

Western Blot using Creatine Kinase BB Polyclonal Antibody (Product # PA5-80476) at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate, Lane B: Hela Whole Cell Lysate, Lane C: A431 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 43 kDa. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Creatine Kinase BB Antibody in Western Blot (WB)
Creatine Kinase BB Antibody in Immunoprecipitation (IP)
Creatine Kinase BB Polyclonal Antibody

Product Details

PA5-80476

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

ELISA (ELISA)

1:5,000-1:10,000
-

Immunoprecipitation (IP)

1-4 µL/mg of lysate
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Human CKB/Creatine kinase B type protein (Pro2-Lys381)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2787789

Product Specific Information

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

This antibody has specificity for Human CKB/Creatine kinase B type.

Target Information

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: B-CK; Brain creatine kinase; CPK-B; Creatine kinase B chain; Creatine kinase B-type; creatine kinase brain; creatine kinase brain-type; creatine kinase, brain; creatine kinase-B; Creatine phosphokinase B-type; epididymis luminal protein 211; epididymis secretory protein Li 29

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Gene Aliases: B-CK; BCK; CKB; CKBB; HEL-211; HEL-S-29

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UniProt ID: (Human) P12277

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Entrez Gene ID: (Human) 1152

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Function(s)
creatine kinase activity protein binding ATP binding ubiquitin protein ligase binding
Process(es)
creatine metabolic process phosphorylation substantia nigra development cellular chloride ion homeostasis
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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