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  • Contactin 2 Antibodies

Invitrogen

Contactin 2 Polyclonal Antibody

View all (16) Contactin 2 antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite Contactin 2 Polyclonal Antibody

  • Antibody Testing Data (1)
Contactin 2 Antibody in Western Blot (WB)
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Contactin 2 Antibody in Western Blot (WB)
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Contactin 2 Antibody (PA5-121133) in WB

Western blot analysis of Contactin 2 in extracts of various cell lines. Samples were incubated with Contactin 2 Polyclonal antibody (Product # PA5-121133) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Contactin 2 Antibody in Western Blot (WB)
Contactin 2 Polyclonal Antibody

Product Details

PA5-121133

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 785-1012 of human Contactin 2 (NP_005067.1).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.37 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2914705

Product Specific Information

Positive test controls include: Mouse brain, Mouse spinal cord, Rat brain. The target is usually found in the following locations: Cell membrane, GPI-anchor, Lipid-anchor.

Immunogen sequence: EVKIRSYNRR GDGPESLTAL VYSAEEEPRV APTKVWAKGV SSSEMNVTWE PVQQDMNGIL LGYEIRYWKA GDKEAAADRV RTAGLDTSAR VSGLHPNTKY HVTVRAYNRA GTGPASPSAN ATTMKPPPRR PPGNISWTFS SSSLSIKWDP VVPFRNESAV TGYKMLYQND LHLTPTLHLT GKNWIEIPVP EDIGHALVQI RTTGPGGDGI PAEVHIVRNG GTSMMVEN

Target Information

Contactin 2 (CNTN2, transiently-expressed axonal glycoprotein, AXT, TAX, TAX1, TAG-1, axonin 1) is a neuronal cell adhesion molecule (CAM) that influences the formation of axon connections in the developing nervous system. Contactin 2 is a member of the immunoglobulin superfamily (IgSF) and contains a glycosylphosphatidylinositol-anchor, six immunoglobulin (Ig)-like and four Fibronectin type III (FNIII)-like domains. Contactin 2 is expressed predominantly during neural development on the cell membrane of axons in nerve fiber tracts in order to guide commissural axons without promoting their growth. Contactin 2 binds with NgCAM in the plane of the same membrane (cis-binding). The Contactin 2 heterophilic (Contactin 2/NgCAM and Contactin 2/NrCAM) binding sites are localized to the first four Ig domains. The Contactin 2 homophilic (Contactin 2/Contactin 2) binding site is localized to the FNIII domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Axonal glycoprotein TAG-1; axonin; Axonin 1; Axonin-1; Axonin1; Contactin-2; Contactin2; DKFZp781D102; MGC157722; TAX-1; Transient axonal glycoprotein 1; transiently-expressed axonal glycoprotein

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Gene Aliases: Cntn2; D130012K04Rik; TAG-1; TAG-564; TAG1; Tax; Tax1

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UniProt ID: (Mouse) Q61330, (Rat) P22063

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Entrez Gene ID: (Mouse) 21367, (Rat) 25356

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Function(s)
glycoprotein binding protein binding carbohydrate binding protein self-association
Process(es)
microtubule cytoskeleton organization neuron migration cell adhesion cell-matrix adhesion axonogenesis axon guidance axonal fasciculation learning adult walking behavior regulation of cell morphogenesis involved in differentiation positive regulation of protein processing cerebral cortex GABAergic interneuron migration central nervous system myelination regulation of axon diameter neuron projection development receptor internalization clustering of voltage-gated potassium channels negative regulation of neuron differentiation regulation of neuronal synaptic plasticity regulation of astrocyte differentiation positive regulation of adenosine receptor signaling pathway protein localization to juxtaparanode region of axon establishment of protein localization to juxtaparanode region of axon presynaptic membrane organization cellular protein localization
It has to be done as per old AB suggested Products section.
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