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  • Primary Antibodies ›
  • Contactin 1 Antibodies

Invitrogen

Contactin 1 Polyclonal Antibody

View all (16) Contactin 1 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Contactin 1 Polyclonal Antibody

  • Antibody Testing Data (2)
Contactin 1 Antibody in Immunocytochemistry (ICC/IF)
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Contactin 1 Antibody in Immunocytochemistry (ICC/IF)
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Contactin 1 Antibody (PA5-143923) in ICC/IF

Immunocytochemistry analysis of Contactin 1 in A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in Contactin 1 Polyclonal antibody (Product # PA5-143923) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Contactin 1 Antibody in Immunocytochemistry (ICC/IF)
Contactin 1 Antibody in Western Blot (WB)
Contactin 1 Polyclonal Antibody

Product Details

PA5-143923

Applications
Tested Dilution
Publications

Western Blot (WB)

0.25-0.5 µg/mL
-

Immunocytochemistry (ICC/IF)

5 µg/mL
-

ELISA (ELISA)

0.1-0.5 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived human Contactin 1/CNTN1 recombinant protein (Position: K225-H979).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3075137

Product Specific Information

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Positive Control - WB: rat brain tissue, mouse brain tissue. ICC/IF: A549 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

Contactin 1 encodes a protein that is a member of the immunoglobulin superfamily. It is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Contactin-1; Glycoprotein gp135; Neural cell surface protein F3

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Gene Aliases: AW495098; CNTN; CNTN1; F3; F3cam; GP135; MYPCN

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UniProt ID: (Human) Q12860, (Rat) Q63198, (Mouse) P12960

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Entrez Gene ID: (Human) 1272, (Rat) 117258, (Mouse) 12805

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Function(s)
glycoprotein binding carbohydrate binding protein binding
Process(es)
cell adhesion Notch signaling pathway positive regulation of gene expression positive regulation of sodium ion transport positive regulation of neuron projection development cerebellum development neuron projection development positive regulation of peptidyl-tyrosine phosphorylation
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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