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  • Collagen IV Antibodies

Invitrogen

Collagen IV Polyclonal Antibody

1 Published Figure
5 References
View all (45) Collagen IV antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Collagen IV Polyclonal Antibody

  • Antibody Testing Data (1)
  • Published Figures (1)
Collagen IV Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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Collagen IV Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Group 53 Created with Sketch.

FIGURE: 1 / 2

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Collagen IV Antibody (PA1-28534) in IHC (P)

Immunohistochemistry analysis of Collagen IV was performed in human kidney, glomeruli and liver tissues using Collagen IV Polyclonal Antibody (Product # PA1-28534) at a dilution of 1:400 for 45 minutes a room temperature. The antibody showed strong staining in FFPE sections of human kidney (Left) with strong red staining observed in glomeruli and liver (Right) with strong staining in sinusoids. Staining for... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Collagen IV Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Collagen IV Antibody in Immunohistochemistry (IHC)
Collagen IV Polyclonal Antibody

Product Details

PA1-28534

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:10,000
View 1 publication 1 publication

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent
View 1 publication 1 publication

Immunoprecipitation (IP)

1:100
View 1 publication 1 publication

Dot blot (DB)

Assay-dependent
-
Product Specifications

Species Reactivity

Bovine, Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Collagen Type IV from human and bovine placenta.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.18 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1956972

Product Specific Information

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Collagen IV is a type of collagen protein that is a major component of the basement membrane in various tissues, including the kidneys, lungs, and skin. It forms a network-like structure that provides support and stability to the surrounding tissue. Collagen IV is composed of three alpha chains, which are encoded by the COL4A1, COL4A2, and COL4A3 genes. Mutations in these genes can lead to various genetic disorders, such as Alport syndrome and Goodpasture syndrome, which affect the kidneys and lungs, respectively.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: arresten; COL4A1 NC1 domain; Collagen 4; Collagen alpha-1(IV) chain; Collagen alpha-2(IV) chain; Collagen alpha-3(IV) chain; Collagen alpha-4(IV) chain; Collagen alpha-5(IV) chain; Collagen alpha-6(IV) chain; collagen IV, alpha-1 polypeptide; collagen IV, alpha-3 polypeptide; Collagen IV, alpha-4 polypeptide; collagen IV, alpha-5 polypeptide; collagen IV, alpha-6 polypeptide; collagen of basement membrane, alpha-1 chain; collagen of basement membrane, alpha-4 chain; collagen of basement membrane, alpha-5 chain; collagen of basement membrane, alpha-6; collagen, type IV, alpha 1; collagen, type IV, alpha 3 (Goodpasture antigen); collagen, type IV, alpha 4; collagen, type IV, alpha 5; collagen, type IV, alpha 6; dA149D17.3; dA24A23.1; dJ889N15.4 (Collagen Alpha 6(IV)); Goodpasture antigen; tumstatin; Type IV Collagen

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Gene Aliases: ASLN; ATS; BOS_12713; BOS_2161; BOS_2253; BSVD; CA44; CA54; COL4A1; COL4A2; COL4A3; COL4A4; COL4A5; COL4A6; CXDELq22.3; DELXq22.3; DFNX6; ICH; POREN2; RATOR

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UniProt ID: (Human) P02462, (Bovine) Q7SIB2, (Human) P08572, (Human) Q01955, (Human) P53420, (Human) P29400, (Human) Q14031

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Entrez Gene ID: (Human) 1282, (Bovine) 282191, (Human) 1284, (Human) 1285, (Bovine) 317711, (Human) 1286, (Bovine) 407107, (Human) 1287, (Human) 1288

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Function(s)
extracellular matrix structural constituent protein binding extracellular matrix constituent conferring elasticity platelet-derived growth factor binding integrin binding structural molecule activity metalloendopeptidase inhibitor activity
Process(es)
extracellular matrix organization collagen catabolic process glomerular basement membrane development activation of cysteine-type endopeptidase activity involved in apoptotic process cell adhesion cell surface receptor signaling pathway sensory perception of sound blood circulation cell proliferation negative regulation of cell proliferation negative regulation of endopeptidase activity negative regulation of angiogenesis endothelial cell apoptotic process patterning of blood vessels brain development neuromuscular junction development epithelial cell differentiation blood vessel morphogenesis retinal blood vessel morphogenesis renal tubule morphogenesis cellular response to amino acid stimulus basement membrane organization
It has to be done as per old AB suggested Products section.
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