Invitrogen

Collagen II Polyclonal Antibody

1 Published Figure
1 Reference
View all (31) Collagen II antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers
Collagen II Polyclonal Antibody

Product Details

PA1-26206

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:10,000
View 1 publication 1 publication

Immunohistochemistry (IHC)

-
- -

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:400
-

Immunocytochemistry (ICC/IF)

Assay-dependent
-

ELISA (ELISA)

1:5,000-1:50,000
-

Immunoprecipitation (IP)

1:100
-

Dot blot (DB)

Assay-dependent
-
Product Specifications

Species Reactivity

Bovine, Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Collagen Type II from human knee cartilage and bovine nasal cartilage.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.03 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 8.0, with 0.150M NaCl

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_779883

Product Specific Information

Recommended positive controls: chondrocyte tissue.

PA1-26206 is expected to cross react with most mammals due to sequence homology. This antibody shows negligable cross reactivity with the Collagens 1, 3, 4, 5, and 6.

Target Information

COL2A1 is the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Alpha-1 type II collagen; arthroophthalmopathy, progressive (Stickler syndrome); cartilage collagen; CGA; chondrocalcin; Collagen 2; Collagen alpha-1(II) chain; collagen II, alpha-1 polypeptide; collagen type II (Col2A1) gene, enhancer region; collagen, type II, alpha 1; cyanogen bromide; MGC131516; pro-alpha 1(II); Procollagen II alpha 1; procollagen, type II, alpha 1

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Gene Aliases: ANFH; AOM; BOS_5148; CB11; CG2A1A; COL11A3; COL2A1; COLLII; SEDC; STL1

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UniProt ID: (Human) P02458, (Bovine) P02459, (Rat) P05539

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Entrez Gene ID: (Human) 1280, (Bovine) 407142, (Rat) 25412

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Function(s)
extracellular matrix structural constituent conferring tensile strength identical protein binding metal ion binding platelet-derived growth factor binding extracellular matrix structural constituent
Process(es)
skeletal system development cartilage condensation tissue homeostasis endochondral ossification chondrocyte differentiation heart morphogenesis growth plate cartilage development proteoglycan metabolic process central nervous system development visual perception sensory perception of sound response to mechanical stimulus response to X-ray regulation of gene expression collagen fibril organization notochord development limb morphogenesis inner ear morphogenesis skeletal system morphogenesis inner ear development cartilage development palate development limb bud formation embryonic skeletal joint morphogenesis bone development cartilage development involved in endochondral bone morphogenesis cellular response to mechanical stimulus cellular response to retinoic acid cellular response to vitamin E cellular response to nicotine cellular response to tumor necrosis factor cellular response to parathyroid hormone stimulus cellular response to peptide hormone stimulus otic vesicle development cellular response to BMP stimulus response to fibroblast growth factor negative regulation of extrinsic apoptotic signaling pathway in absence of ligand extracellular matrix organization collagen catabolic process regulation of immune response negative regulation of apoptotic process
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