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  • Chordin Antibodies

Invitrogen

Chordin Polyclonal Antibody

View all (9) Chordin antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite Chordin Polyclonal Antibody

  • Antibody Testing Data (1)
Chordin Antibody in Western Blot (WB)
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Chordin Antibody in Western Blot (WB)
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Chordin Antibody (PA5-109536) in WB

Western blot analysis of Chordin in extracts of various cell lines. Samples were incubated with Chordin Polyclonal antibody (Product # PA5-109536) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Chordin Antibody in Western Blot (WB)
Chordin Polyclonal Antibody

Product Details

PA5-109536

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 686-955 of human CHRD (NP_003732.2).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.32 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2854947

Product Specific Information

Immunogen sequence: PALAPAKPGG PGRPRDPNTC FFEGQQRPHG ARWAPNYDPL CSLCTCQRRT VICDPVVCPP PSCPHPVQAP DQCCPVCPEK QDVRDLPGLP RSRDPGEGCY FDGDRSWRAA GTRWHPVVPP FGLIKCAVCT CKGGTGEVHC EKVQCPRLAC AQPVRVNPTD CCKQCPVGSG AHPQLGDPMQ ADGPRGCRFA GQWFPESQSW HPSVPPFGEM SCITCRCGAG VPHCERDDCS LPLSCGSGKE SRCCSRCTAH RRPAPETRTD PELEKEAEGS

Target Information

This gene encodes a secreted protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta-like bone morphogenetic proteins and sequestering them in latent complexes. The encoded protein may also have roles in organogenesis and during adulthood. Multiple transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants have been described but their full length sequence has not been determined.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Chordin

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Gene Aliases: Chd; Chrd

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UniProt ID: (Mouse) Q9Z0E2

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Entrez Gene ID: (Rat) 117275, (Mouse) 12667

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Function(s)
heparin binding syndecan binding protein binding
Process(es)
skeletal system development osteoblast differentiation gastrulation with mouth forming second mesoderm formation positive regulation of mesenchymal cell proliferation pattern specification process central nervous system development dorsal/ventral pattern formation BMP signaling pathway involved in spinal cord dorsal/ventral patterning negative regulation of cell migration negative regulation of BMP signaling pathway forebrain development negative regulation of osteoblast differentiation positive regulation of cell adhesion multicellular organism development
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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