Invitrogen

Cdc37 Polyclonal Antibody

Name: Cdc37 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-78357

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View all (28) CDC37 antibodies

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Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)
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FIGURE: 1 / 5

Cdc37 Antibody (PA5-78357) in WB

Western blot was performed using Anti-Cdc37 Polyclonal Antibody (Product # PA5-78357) and a ~50 kDa band corresponding to CDC37 was observed across the cell lines tested. Whole cell extracts (30 µg lysate) of A-431 (Lane 1), HeLa (Lane2), Jurkat (Lane 3), HEL 92.1.7 (Lane 4) and HEK-293 (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed... View More

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Cdc37 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A-431 cells were transfected with CDC37 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-Cdc37 Polyclonal Antibody (Product # PA5-78357). {KD}

Product Details

PA5-78357

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human CDC37. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.97 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2735619

Product Specific Information

Positive Control: Jurkat, Raji, NCI-H929

Predicted Reactivity: Mouse (95%), Rat (90%), Pig (97%), Chicken (85%), Bovine (98%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

In S. cerevisiae, Cdc37 has been shown to be an important regulator of cell division cycle through its action on cyclin-dependent kinases (CDK's). Cdc37 has also been associated with signaling pathways and kinases unrelated to cell cycle control, including v-Src, casein kinase II, MPS1 kinase and the sevenless receptor tyrosine kinase. Interestingly, HSP90 has also been shown to be associated with many of the same cellular targets as Cdc37. It has now been shown that Cdc37 is the same protein previously described as p50 which has been found to be associated with many of the same proteins as HSP90. Among others, Cdc37/p50 has been found complexed with Src-family kinases (Fyn, Yes, Fes, Lck & Fgr), Raf and pp60v-src. More recently, Cdc37 has been shown to be a chaperone independently and in concert with HSP90. In fact, Cdc37 is necessary for the binding of HSP90 to Cdk4.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CDC 37; CDC37 (cell division cycle 37, S. cerevisiae, homolog); CDC37 cell division cycle 37 homolog; Cdc37 homolog; CDC37 protein; cell division cycle 37 homolog; Hsp90 chaperone protein kinase-targeting subunit; Hsp90 co-chaperone Cdc37; p50Cdc37

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Gene Aliases: CDC37; CDC37A; p50; P50CDC37

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UniProt ID: (Human) Q16543, (Mouse) Q61081

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Entrez Gene ID: (Human) 11140, (Mouse) 12539

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Function(s)

protein tyrosine kinase activity protein binding protein kinase regulator activity kinase binding protein kinase binding heat shock protein binding unfolded protein binding chaperone binding Hsp90 protein binding protein C-terminus binding mitogen-activated protein kinase kinase kinase binding protein kinase B binding

Process(es)

regulation of cyclin-dependent protein serine/threonine kinase activity protein folding protein targeting posttranscriptional regulation of gene expression peptidyl-tyrosine phosphorylation ERBB2 signaling pathway protein stabilization regulation of interferon-gamma-mediated signaling pathway regulation of type I interferon-mediated signaling pathway mitophagy in response to mitochondrial depolarization regulation of protein kinase activity

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cdc37 Polyclonal Antibody

Cdc37 Antibody (PA5-78357) in WB

Product Details

PA5-78357

Western Blot (WB)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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