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  • Primary Antibodies ›
  • CDC25C Antibodies

Invitrogen

Cdc25C Recombinant Rabbit Monoclonal Antibody (3E6)

Advanced Verification
View all (35) CDC25C antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Cdc25C Recombinant Rabbit Monoclonal Antibody (3E6)

  • Antibody Testing Data (6)
  • Advanced Verification (1)
Cdc25C Antibody in Immunocytochemistry (ICC/IF)
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Cdc25C Antibody in Immunocytochemistry (ICC/IF)
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Cdc25C Antibody (MA5-33127) in ICC/IF

Immunofluorescence analysis of M-phase inducer phosphatase 3 was performed using 70% confluent log phase HCT 116 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Cdc25C Recombinant Rabbit Monoclonal Antibody (Product # MA5-33127) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Cdc25C Antibody in Immunocytochemistry (ICC/IF)
Cdc25C Antibody in Immunocytochemistry (ICC/IF)
Cdc25C Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Cdc25C Antibody in Western Blot (WB)
Cdc25C Antibody in Western Blot (WB)
Cdc25C Antibody in Western Blot (WB)
Cdc25C Antibody
Cdc25C Recombinant Rabbit Monoclonal Antibody (3E6)

Product Details

MA5-33127

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:5,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:20-1:200
-

ELISA (ELISA)

Assay-Dependent
-

Immunoprecipitation (IP)

1:200-1:1,000
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

3E6

Immunogen

A synthesized peptide derived from human CDC25C
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.5 mg/mL

Purification

Protein G, Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2811943

Target Information

M-phase inducer phosphatase 3is an enzyme that in humans is encoded by theCDC25Cgene. This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 (CDK1) and triggers entry into mitosis. Also, it is thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Dual specificity phosphatase Cdc25C; EC 3.1.3.48; M-phase inducer phosphatase 3; mitosis inducer CDC25; MPIP3; OTTHUMP00000159490; OTTHUMP00000224012; OTTHUMP00000224013; OTTHUMP00000224016; phosphotyrosine phosphatase; protein phosphatase 1, regulatory subunit 60

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Gene Aliases: CDC25; CDC25C; PPP1R60

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UniProt ID: (Human) P30307

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Entrez Gene ID: (Human) 995

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Function(s)
phosphoprotein phosphatase activity protein tyrosine phosphatase activity protein binding protein kinase binding WW domain binding
Process(es)
regulation of cyclin-dependent protein serine/threonine kinase activity G2/M transition of mitotic cell cycle DNA replication DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest mitotic nuclear division regulation of mitotic nuclear division spermatogenesis cell proliferation viral process peptidyl-tyrosine dephosphorylation cell division regulation of cell cycle
It has to be done as per old AB suggested Products section.
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