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  • CXCL12 alpha Antibodies

Invitrogen

CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody, PeproTech®

8 References
View all (7) CXCL12 alpha antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody, PeproTech®

  • Antibody Testing Data (3)
CXCL12 alpha (SDF-1 alpha) Antibody in ELISA (ELISA)
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CXCL12 alpha (SDF-1 alpha) Antibody in ELISA (ELISA)
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CXCL12 alpha (SDF-1 alpha) Antibody (500-P164G-1MG) in ELISA

Sandwich ELISA: To detect Murine SDF-1alpha (CXCL12) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody (Product # 500-P164G-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody, Biotin (Product # 500-P164GBT-1MG) as a detection antibody, ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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CXCL12 alpha (SDF-1 alpha) Antibody in Western Blot (WB)
CXCL12 alpha (SDF-1 alpha) Antibody in Western Blot (WB)
CXCL12 alpha (SDF-1 alpha) Antibody in ELISA (ELISA)
CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody, PeproTech®

Product Details

500-P164G-1MG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
View 2 publications 2 publications

ELISA (ELISA)

0.5-2.0 µg/mL
View 1 publication 1 publication

In vitro Assay (IV)

-
View 2 publications 2 publications

Immunostaining (IS)

-
View 3 publications 3 publications
Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Goat

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Murine SDF-1alpha (CXCL12)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929582

Product Specific Information

AA Sequence of recombinant protein: KPVSLSYRCP CRFFESHIAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK.

Preparation: Produced from sera of goats immunized with highly pure recombinant Murine SDF-1alpha (CXCL12). Anti-Murine SDF-1alpha (CXCL12)-specific antibody was purified by affinity chromatography employing an immobilized Murine SDF-1alpha (CXCL12) matrix.

Sandwich ELISA: To detect Murine SDF-1alpha (CXCL12) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine SDF-1alpha (CXCL12) (500-P164GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine SDF-1alpha (CXCL12).

Western Blot: To detect Murine SDF-1alpha (CXC12) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine SDF-1alpha (CXCL12) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

CXCL12 is a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of some cancers such as breast cancer. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 12-O-tetradecanoylphorbol 13-acetate repressed protein 1; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 12; CC motif chemokine; CCmotif chemokine; CXC; CXC motif chemokine; CXCL; H-SDF-1-alpha; PBSF; Pre-B cell growth-stimulating factor; pre-B-cell growth-stimulating factor; SDF 1 a; SDF 1 alpha; SDF 1α; SDF-1; SDF1 alpha; SDF1α; Stromal cell-derived factor 1; stromal cell-derived factor-1 alpha; Thymic lymphoma cell-stimulating factor; TLSF; TPAR1

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Gene Aliases: Cxcl12; Pbsf; Scyb12; Sdf1; Tlsf; Tpar1

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UniProt ID: (Mouse) P40224

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Entrez Gene ID: (Mouse) 20315

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Function(s)
cytokine activity chemokine activity growth factor activity chemokine receptor binding CXCR chemokine receptor binding
Process(es)
patterning of blood vessels ameboidal-type cell migration neuron migration positive regulation of endothelial cell proliferation chemotaxis immune response germ cell development brain development motor neuron axon guidance positive regulation of cell proliferation adult locomotory behavior germ cell migration telencephalon cell migration regulation of cell migration positive regulation of cell migration organ regeneration positive regulation of dopamine secretion T cell proliferation positive regulation of neuron differentiation positive regulation of cell adhesion positive regulation of axon extension involved in axon guidance induction of positive chemotaxis regulation of calcium ion transport cell chemotaxis chemokine-mediated signaling pathway positive regulation of monocyte chemotaxis positive regulation of myoblast fusion negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage negative regulation of leukocyte tethering or rolling negative regulation of leukocyte apoptotic process positive regulation of T cell migration
It has to be done as per old AB suggested Products section.
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