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  • COL6A1 Antibodies

Invitrogen

COL6A1 Polyclonal Antibody

Advanced Verification
View all (15) COL6A1 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite COL6A1 Polyclonal Antibody

  • Antibody Testing Data (7)
  • Advanced Verification (2)
COL6A1 Antibody in Immunocytochemistry (ICC/IF)
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COL6A1 Antibody in Immunocytochemistry (ICC/IF)
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COL6A1 Antibody (PA5-29068) in ICC/IF

Immunofluorescence analysis of COL6A1 was performed using 70% confluent log phase IMR-90 and SK-O-V3 cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 5 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with COL6A1 Polyclonal Antibody (Product # PA5-29068) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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COL6A1 Antibody in Immunocytochemistry (ICC/IF)
COL6A1 Antibody in Immunocytochemistry (ICC/IF)
COL6A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
COL6A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
COL6A1 Antibody in Western Blot (WB)
COL6A1 Antibody in Western Blot (WB)
COL6A1 Antibody in Western Blot (WB)
COL6A1 Antibody
COL6A1 Antibody

Product Details

PA5-29068

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human COL6A1. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2546544

Product Specific Information

Recommended positive controls: 293T, U87-MG.

Predicted reactivity: Mouse (85%), Bovine (87%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (typesI, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: alpha 1 (VI) chain (61 AA); Collagen alpha-1(VI) chain; collagen VI, alpha-1 polypeptide; collagen, type VI, alpha 1; procollagen, type VI, alpha 1

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Gene Aliases: AI747156; BTHLM1; Col6a-1; COL6A1; OPLL; RGD1565398; UCHMD1

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UniProt ID: (Human) P12109, (Mouse) Q04857

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Entrez Gene ID: (Human) 1291, (Mouse) 12833, (Rat) 294337

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Function(s)
platelet-derived growth factor binding molecular_function
Process(es)
osteoblast differentiation cell adhesion protein heterotrimerization cellular response to amino acid stimulus biological_process endodermal cell differentiation extracellular matrix organization collagen catabolic process
It has to be done as per old AB suggested Products section.
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