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Invitrogen
CNTF Polyclonal Antibody, PeproTech®
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FIGURE: 1 / 3
CNTF Antibody (500-P140-1MG) in ELISA
Product Details
500-P140-1MG
Species Reactivity
Published species
Host/Isotype
Class
Type
Immunogen
Conjugate
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Concentration
Purification
Storage buffer
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RRID
Product Specific Information
AA Sequence of recombinant protein: AFTEHSPLTP HRRDLCSRSI WLARKIRSDL TALTQSYVKH QGLNKNINLD SADGMPVAST DQWSQLTQAQ RLQQNLQAYR TFHVLLARLL QDQQVHFTPT QGDFHQAIHT LLLQVAAFAY QIQQLMILLQ YKIPRNQADG MPINVGDGGL FQKKLWGLKV LQQLSQWTVR SIHDLRFISS HQTGIPARGS HYIANNKKM.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human CNTF. Anti-Human CNTF-specific antibody was purified by affinity chromatography employing an immobilized Human CNTF matrix.
Sandwich ELISA: To detect Human CNTF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human CNTF (500-P140Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human CNTF.
Western Blot: To detect Human CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human CNTF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of ZFP91 and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Ciliary neurotrophic factor; CNTF; H-CNTF
Gene Aliases: CNTF; HCNTF
UniProt ID: (Human) P26441
Entrez Gene ID: (Human) 1270
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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