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  • CHI3L1 Antibodies

Invitrogen

CHI3L1 Polyclonal Antibody

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Questions & Answers

Cite CHI3L1 Polyclonal Antibody

  • Antibody Testing Data (1)
CHI3L1 Antibody in Western Blot (WB)
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CHI3L1 Antibody in Western Blot (WB)
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CHI3L1 Antibody (PA5-79042) in WB

Western blot analysis of CHI3L1 in Lane 1: mouse RAW264.7 whole cell lysate, Lane 2: mouse HEPA1-6 whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with CHI3L1 polyclonal antibody (Product # PA5-79042) at a dilution of 0.5 µg/mL (overnight at 4°C),... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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CHI3L1 Antibody in Western Blot (WB)
CHI3L1 Polyclonal Antibody

Product Details

PA5-79042

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-

ELISA (ELISA)

0.1-0.5 µg/mL
-
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived mouse CHI3L1 recombinant protein (Position: Y30-K262).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

0.05mg sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2746158

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: mouse RAW2647 whole cell, mouse HEPA1-6 whole cell.

Target Information

Human cartilage glycoprotein 39 (GP-39), also known as YKL-40, is a glycoprotein secreted by articular chondrocytes, synoviocytes and macrophages. Serum and synovial fluid GP-39 levels are elevated in inflammatory diseases and correlate with the degree of joint destruction in rheumatoid arthritis. GP-39 is expressed in articular chondrocytes and synovial cells, as well as in liver, but is undetectable in muscle tissues, lung, pancreas, mononuclear cells and fibroblasts. GP-39 is a candidate autoantigen in rheumatoid arthritis and is important in the capacity of cells to respond to and cope with changes in their environment.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Breast regression protein 39; BRP39 protein; Cartilage glycoprotein 39; CGP-39; CGP39; Chitinase 3 like protein 1CGP 39; chitinase 3-like 1; Chitinase-3-like protein 1; DKFZp686N19119; FLJ38139; GP-39; hCGP39

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Gene Aliases: AW208766; Brp39; Chi3l1; Chil1; Gp39

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UniProt ID: (Mouse) Q61362

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Entrez Gene ID: (Mouse) 12654

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Function(s)
hydrolase activity, hydrolyzing O-glycosyl compounds chitinase activity chitin binding
Process(es)
carbohydrate metabolic process chitin catabolic process apoptotic process inflammatory response activation of NF-kappaB-inducing kinase activity positive regulation of peptidyl-threonine phosphorylation lung development positive regulation of angiogenesis positive regulation of protein kinase B signaling positive regulation of ERK1 and ERK2 cascade interleukin-8 secretion
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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