Invitrogen

CDK2 Monoclonal Antibody (5B12D1)

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Datasheet
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Datasheet
Protocols
Questions & Answers
CDK2 Monoclonal Antibody (5B12D1)

Product Details

MA5-49149

Applications
Tested Dilution
Publications

Western Blot (WB)

0.25-0.5 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

2-5 µg/mL
-

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

5B12D1

Immunogen

E.coli-derived human Cdk2 recombinant protein (Position: E81-L298).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3074807

Product Specific Information

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Human Cdk2 shares 98.6% amino acid (aa) sequence identity with rat Cdk2.

Positive Control - WB: human Jurkat whole cell, human HepG2 whole cell, human K562 whole cell, rat heart tissue, rat L6 whole cell, mouse heart tissue, mouse C2C12 whole cell. IHC: human breast cancer tissue, human laryngeal squamous cell carcinomas tissue, human liver cancer tissue. Flow: ANA-1 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

CDK2/cyclinO is a member of the cyclin dependent protein kinase family complexed to CyclinO. CyclinO was used as a specific protein isoform name for the UNG gene which is localized exclusively in the nucleus. In addition, CyclinO was reported to shows significant uracil-DNA glycosylase activity. CDK2/cyclinO protein levels increase during G1 phase and the protein is turned over during the course of cell cycle. The levels of cyclin A-cdk2 is maximal at the G1/S transition and both cdk2 and cyclin A are found associated with DNA in the initiation complex during replication. Rb protein acts as substrate for cdk2-cyclin E and/or cdk2-cyclin A in vivo. Cdk2 is activated and specifically localized to the nucleus during late G1-, S-, and G2-phase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cdc2-related protein kinase; CDC28; CDC2A; CDK1; Cell division protein kinase 2; cyclin dependent kinase 2-alpha; Cyclin-dependent kinase 2; EC 2.7.11.22; EC 2.7.11.23; kinase Cdc2; MPF; p33 protein kinase; p33CDK2; p34 protein kinase

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Gene Aliases: A630093N05Rik; CDK2; CDKN2; p33(CDK2)

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UniProt ID: (Human) P24941, (Mouse) P97377

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Entrez Gene ID: (Human) 1017, (Rat) 362817, (Mouse) 12566

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Function(s)
protein serine/threonine kinase activity cyclin-dependent protein serine/threonine kinase activity protein binding ATP binding kinase activity cyclin binding protein complex binding histone kinase activity metal ion binding protein kinase activity nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups
Process(es)
G1/S transition of mitotic cell cycle lens development in camera-type eye DNA repair protein phosphorylation potassium ion transport mitotic nuclear division Ras protein signal transduction positive regulation of cell proliferation response to toxic substance histone phosphorylation peptidyl-serine phosphorylation organ regeneration positive regulation of DNA-dependent DNA replication initiation response to estradiol cellular response to insulin stimulus response to drug response to ethanol positive regulation of transcription, DNA-templated response to cadmium ion cell division meiotic cell cycle response to cAMP response to electrical stimulus regulation of cell cycle regulation of gene silencing cellular response to DNA damage stimulus cell cycle phosphorylation G2/M transition of mitotic cell cycle DNA replication DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest centriole replication meiotic nuclear division mitotic G1 DNA damage checkpoint centrosome duplication negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle cellular response to nitric oxide regulation of signal transduction by p53 class mediator
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