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  • CD70 Antibodies

Invitrogen

CD70 Recombinant Rabbit Monoclonal Antibody (7F4S5)

View all (46) CD70 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite CD70 Recombinant Rabbit Monoclonal Antibody (7F4S5)

  • Antibody Testing Data (4)
CD70 Antibody in Immunocytochemistry (ICC/IF)
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CD70 Antibody in Immunocytochemistry (ICC/IF)
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CD70 Antibody (MA5-42758) in ICC/IF

Immunofluorescence analysis of CD70 was performed using 70% confluent log phase SK-O-V3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD70 Recombinant Rabbit Monoclonal Antibody (7F4S5) (Product # MA5-42758, 1:100) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy chain), Superclonal™ Recombinant Secondary Antibody, Alexa Flu... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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CD70 Antibody in Immunocytochemistry (ICC/IF)
CD70 Antibody in Western Blot (WB)
CD70 Antibody in Western Blot (WB)
CD70 Antibody in Flow Cytometry (Flow)
CD70 Recombinant Rabbit Monoclonal Antibody (7F4S5)

Product Details

MA5-42758

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunocytochemistry (ICC/IF)

1:100
-

Flow Cytometry (Flow)

1:100-1:800
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

7F4S5

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 39-193 of human CD70 (P32970)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2911899

Product Specific Information

Positive test controls include: Raji, 293T.

Immunogen sequence: QRFAQAQQQL PLESLGWDVA ELQLNHTGPQ QDPRLYWQGG PALGRSFLHG PELDKGQLRI HRDGIYMVHI QVTLAICSST TASRHHPTTL AVGICSPASR SISLLRLSFH QGCTIASQRL TPLARGDTLC TNLTGTLLPS RNTDETFFGV QWVRP

Target Information

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin synthesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD27 ligand; CD27-L; CD70; CD70 antigen; Ki-24 antigen; surface antigen CD70; tumor necrosis factor (ligand) superfamily, member 7; tumor necrosis factor ligand 8A; Tumor necrosis factor ligand superfamily member 7

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Gene Aliases: CD27L; CD27LG; CD70; TNFSF7; TNLG8A

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UniProt ID: (Human) P32970

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Entrez Gene ID: (Human) 970

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Function(s)
protease binding receptor binding cytokine activity tumor necrosis factor receptor binding protein binding
Process(es)
immune response signal transduction cell-cell signaling cell proliferation tumor necrosis factor-mediated signaling pathway extrinsic apoptotic signaling pathway
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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