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Invitrogen
CD235a (Glycophorin A) Monoclonal Antibody (HIR2 (GA-R2)), APC, eBioscience™
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-Flow-20170216130641.jpg?time=20250104 "CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)")
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-Flow-20170216130641.jpg?time=20250104?time=20250104 "CD235a (Glycophorin A) Antibody (17-9987-42) in Flow")
Product Details
17-9987-42
Species Reactivity
Published species
Host/Isotype
Recommended Isotype Control
Class
Type
Clone
Conjugate
Excitation/Emission Max
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Purification
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Contains
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RRID
Product Specific Information
Description: The HIR2 monoclonal antibody reacts with human glycophorin A, a sialoglycoprotein expressed by erythroid precursors and mature circulating red cells.
Applications Reported: This HIR2 (also GA-R2) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This HIR2 (also GA-R2) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.015 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Glycophorin A, also known as CD235a, is a 151 amino acid sialoglycoprotein expressed on the membrane of mature erythrocytes and erythroid progenitor cells, with approximately 500,000 copies per cell. The gene for glycophorin A is located on chromosome 4 and exists in two allelic forms, M and N, which differ by two amino acids: the M group has Ser1 and Gly5, while the N group has Leu1 and Glu5. These allelic variations define the blood group M and N specificities. Glycophorin A serves multiple functions, including providing a mucin-like barrier that minimizes aggregation between red blood cells in circulation, potentially preventing cell fusion. It also acts as a receptor for certain pathogens, including Sandei virus, parvovirus, and Hsa, a Streptococcus adhesin. Recent studies suggest that exposure to toxins can lead to mutations or loss of alleles, resulting in phenotypic changes. There is ongoing research correlating genotype and phenotype with predisposition to diseases such as cancer and heart disease, highlighting the importance of glycophorin A in both health and disease. Glycophorin A is a significant marker for studying erythrocyte-related functions and pathologies, as well as its role in pathogen interactions and disease predisposition.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD235; CD235a; erythroid-lineage-specific membrane sialoglycoprotein; glycophorin A (MN blood group); glycophorin A, GPA; glycophorin Erik; glycophorin MiI; glycophorin MiV; glycophorin SAT; glycophorin Sta type C; Glycophorin-A; HGNC:4702; HGpMiX; HGpStaC; Mi.V glycoprotein; Mi.V glycoprotein (24 AA); MN sialoglycoprotein; PAS-2; recombinant glycophorin A-B Miltenberger-DR; Sialoglycoprotein alpha
Gene Aliases: CD235a; GPA; GPErik; GPSAT; GYPA; HGpMiV; HGpMiXI; HGpSta(C); MN; MNS; PAS-2
UniProt ID: (Human) P02724
Entrez Gene ID: (Human) 2993
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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