Invitrogen

CD171 Monoclonal Antibody (UJ127.11)

Name: CD171 Monoclonal Antibody (UJ127.11)
Brand: Invitrogen
SKU: MA1-46044

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View all (90) CD171 (L1CAM) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (5)
Advanced Verification (1)

CD171 Antibody in Immunocytochemistry (ICC/IF)

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CD171 Antibody (MA1-46044) in ICC/IF

Immunocytochemistry analysis of CD171 in Neuro2A cells. Samples were incubated with CD171 monoclonal antibody (Product # MA1-46044) using a dilution of 5 µg/mL overnight at 4°C followed by anti-mouse Dylight 488 (Green) at a dilution of 1:1,000 dilution for 60 minutes. The left panel (A) shows untreated Neuro2a cells and the right panel (B) shows Neuro2a cells that were serum starved then treated with 1mM cAMP overnight to induce axon growth. Cells were fixed in 4% paraformaldehyde for 10 minutes and then permeabilized for 5 minutes using ... View More

CD171 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

CD171 Antibody — Fig. 5. Changes in the CSF levels of protein mediators of neurodevelopment after initiation PHH treatment are protein-specific. A, Volcano plot showing changes in the relative abundances of CSF protein mediators of neurodevelopment identified with tandem MAF proteomics after initiation of PHH treatment. The p values from the Benjamini-Hochberg-corrected ANOVA results are plotted against the log2 fold change. The proteins shown are the quantifiable proteins from the neurodevelopmental pathway shown in Fig. 1. After initiation of PHH treatment, proteins displayed a decrease (left of 0 on the axis), increase (right of 0), or no significant change at all (below the hashed red line). Note the decrease in APP and L1CAM over the 7 day interval from S1 to S2, whereas ActB and ApoA4 increase. Fold-change measurements for the proteins demonstrating a significant difference between samples are shown in Table II. B, Conventional laboratory techniques including Western blot (L1CAM, APP, and ActB) and ELISA (ApoA4) confirm the trends in protein levels observed with tandem MAF process. The immunoblot for beta actin was shown in the previous figure. Asterisks denote p < 0.05, n = 3 per group. {TM}

Immunogen image

Immunogen Image CD171 Monoclonal Antibody (UJ127.11)

Product Details

MA1-46044

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

2-5 µg/mL

Flow Cytometry (Flow)

2.5 µg/mL

ELISA (ELISA)

1:100-1:2,000

Immunoprecipitation (IP)

1:10-1:500

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

UJ127.11

Immunogen

Homogenous suspension of 16 week human fetal brain.

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A/G

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1016758

Target Information

L1CAM/CD171 is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: antigen identified by monoclonal antibody R1; CD 171; CD171; CD171 molecule; N-CAM-L1; Neural cell adhesion molecule L1; sCD171; sL1 CAM; sL1CAM; soluble CD 171; soluble CD171; soluble L1 CAM; soluble L1CAM

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Gene Aliases: CAML1; CD171; HSAS; HSAS1; L1; L1CAM; MASA; MIC5; N-CAM-L1; N-CAML1; NCAM-L1; S10; SPG1

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UniProt ID: (Human) P32004

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Entrez Gene ID: (Human) 3897, (Mouse) 16728

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Function(s)

integrin binding protein binding PDZ domain binding sialic acid binding identical protein binding protein self-association

Process(es)

chemotaxis cell adhesion nervous system development axon guidance positive regulation of axon extension leukocyte migration homophilic cell adhesion via plasma membrane adhesion molecules heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules leukocyte cell-cell adhesion cell surface receptor signaling pathway multicellular organism development positive regulation of cell-cell adhesion cell differentiation neuron projection development cell-cell adhesion mediated by integrin homotypic cell-cell adhesion positive regulation of calcium-mediated signaling

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD171 Monoclonal Antibody (UJ127.11)

CD171 Antibody (MA1-46044) in ICC/IF

Product Details

MA1-46044

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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