Disclaimer

Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

  • Primary Antibodies ›
  • CD166 (ALCAM) Antibodies

Invitrogen

CD166 Recombinant Rabbit Monoclonal Antibody (001)

View all (62) CD166 (ALCAM) antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite CD166 Recombinant Rabbit Monoclonal Antibody (001)

  • Antibody Testing Data (2)
CD166 Antibody in Western Blot (WB)
Group 53 Created with Sketch.
CD166 Antibody in Western Blot (WB)
Group 53 Created with Sketch.

FIGURE: 1 / 2

{{ $ctrl.currentElement.advancedVerification.fullName }}
{{ $ctrl.currentElement.advancedVerification.text }}

CD166 Antibody (MA5-29859) in WB

Western Blot analysis of CD166 (ALCAM) using CD166 Recombinant Monoclonal (Product # MA5-29859) at a 1:500 dilution. Sample: Lysates/proteins at 30 μg per lane. Lane A: SH-SY5Y whole cell lysate; Lane B: Daudi whole cell lysate; Lane C: HuT78 whole cell lysate. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:65 kDa, Observed band size:100 kDa. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
Published figure supplied by benchsci-logo
PMID: {{$ctrl.currentElement.benchSciPubmedId}}
View Product

{{$ctrl.videoDesc}}

{{$ctrl.videoLongDesc}}

CD166 Antibody in Western Blot (WB)
CD166 Antibody in Immunoprecipitation (IP)
CD166 Recombinant Rabbit Monoclonal Antibody (001)

Product Details

MA5-29859

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

ELISA (ELISA)

1:5,000-1:10,000
-

Immunoprecipitation (IP)

1-4 µL/mg of lysate
-
Product Specifications

Species Reactivity

Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

001

Immunogen

Recombinant Rat CD166/ALCAM protein (Met1-Lys527)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2785676

Product Specific Information

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

This antibody has specificity for Rat CD166/ALCAM.

Target Information

This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: Activated leukocyte cell adhesion molecule; CD166; CD166 antigen; FLJ38514; HB2; KG-CAM; MGC71733; Protein MEMD; SB-10 antigen

View more View less

Gene Aliases: Alcam

View more View less

UniProt ID: (Rat) O35112

View more View less

Entrez Gene ID: (Rat) 79559

View more View less

Process(es)
cell adhesion axon guidance motor neuron axon guidance
It has to be done as per old AB suggested Products section.
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support

Your items have has been added!


Host server : magellan-search-55c564d944-rwwh4:80/100.66.128.142:80.
git-commit: 3092084cb0e494f0e07f07633350183de7fa10a8
git-url: https://github.com/thermofisher/magellan-search
git-branch: release/2.36.2-2025.09.39-1.0