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  • CD166 (ALCAM) Antibodies

Invitrogen

CD166 (ALCAM) Recombinant Rabbit Monoclonal Antibody (6Z4Y2)

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Datasheet
Protocols
Questions & Answers

Cite CD166 (ALCAM) Recombinant Rabbit Monoclonal Antibody (6Z4Y2)

  • Antibody Testing Data (1)
CD166 (ALCAM) Antibody in Western Blot (WB)
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CD166 (ALCAM) Antibody in Western Blot (WB)
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CD166 (ALCAM) Antibody (MA5-38206) in WB

Western blot analysis of CD166 (ALCAM) in extracts of various cell lines. Samples were incubated with CD166 (ALCAM) Monoclonal antibody (Product # MA5-38206) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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CD166 (ALCAM) Antibody in Western Blot (WB)
CD166 (ALCAM) Recombinant Rabbit Monoclonal Antibody (6Z4Y2)

Product Details

MA5-38206

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

6Z4Y2

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 484-583 of human CD166/ALCAM (Q13740)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.8 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2898123

Product Specific Information

Positive Samples: THP-1, SH-SY5Y, Mouse lung, Mouse brain, Rat lung, Rat liver, Rat brain

Immunogen sequence: TCTAENQLER TVNSLNVSAI SIPEHDEADE ISDENREKVN DQAKLIVGIV VGLLLAALVA GVVYWLYMKK SKTASKHVNK DLGNMEENKK LEENNHKTEA

Target Information

This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Activated leukocyte cell adhesion molecule; BEN; CD166; CD166 antigen; FLJ38514; HB2; KG-CAM; MGC71733; Protein DM-GRASP; Protein MEMD; SB-10 antigen

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Gene Aliases: AI853494; ALCAM; BEN; CD166; DM-GRASP; MEMD; MuSC; SC1

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UniProt ID: (Human) Q13740, (Rat) O35112, (Mouse) Q61490

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Entrez Gene ID: (Human) 214, (Rat) 79559, (Mouse) 11658

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Function(s)
receptor binding protein binding
Process(es)
adaptive immune response cell adhesion heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules signal transduction motor neuron axon guidance retinal ganglion cell axon guidance axon extension involved in axon guidance neuron projection extension axon guidance
It has to be done as per old AB suggested Products section.
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