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  • CD154 (CD40 Ligand) Antibodies

Invitrogen

CD154 (CD40 Ligand) Recombinant Rabbit Monoclonal Antibody (5H4L1)

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Datasheet
Protocols
Questions & Answers

Cite CD154 (CD40 Ligand) Recombinant Rabbit Monoclonal Antibody (5H4L1)

  • Antibody Testing Data (1)
CD154 (CD40 Ligand) Antibody in Western Blot (WB)
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CD154 (CD40 Ligand) Antibody in Western Blot (WB)
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CD154 (CD40 Ligand) Antibody (MA5-35205) in WB

Western blot analysis of CD154 (CD40 Ligand) in extracts of various cell lines. Samples were incubated with CD154 (CD40 Ligand) Monoclonal antibody (Product # MA5-35205) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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CD154 (CD40 Ligand) Antibody in Western Blot (WB)
CD154 (CD40 Ligand) Recombinant Rabbit Monoclonal Antibody (5H4L1)

Product Details

MA5-35205

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

5H4L1

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-150 of human CD40L (P29965)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.75 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2849109

Product Specific Information

Immunogen sequence: LDKIEDERNL HEDFVFMKTI QRCNTGERSL SLLNCEEIKS QFEGFVKDIM LNKEETKKEN SFEMQKGDQN PQIAAHVISE ASSKTTSVLQ WAEKGYYTMS N

Target Information

CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD154; CD40 ligand; CD40 ligand CD154; CD40-L; H-CD-40-L; M-CD-40-L; RP23-153G22.3; T-cell antigen Gp39; TNF-related activation protein; TRAP; tumor necrosis factor (ligand) superfamily, member 5; Tumor necrosis factor ligand superfamily member 5

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Gene Aliases: CD154; CD40-L; Cd40l; Cd40lg; gp39; HIGM1; IGM; IMD3; Ly-62; Ly62; T-BAM; Tnfsf5; TRAP

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UniProt ID: (Mouse) P27548, (Rat) Q9Z2V2

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Entrez Gene ID: (Mouse) 21947, (Rat) 84349

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Function(s)
cytokine activity tumor necrosis factor receptor binding CD40 receptor binding molecular_function
Process(es)
positive regulation of immune response to tumor cell inflammatory response immune response signal transduction platelet activation B cell differentiation positive regulation of B cell proliferation positive regulation of interferon-gamma production positive regulation of interleukin-10 production positive regulation of interleukin-12 production B cell proliferation negative regulation of apoptotic process isotype switching positive regulation of MHC class II biosynthetic process immunoglobulin secretion regulation of immunoglobulin secretion dendritic cell differentiation positive regulation of endothelial cell apoptotic process
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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